Sektion für Molekularbiologie

hubertus_haas

Molecular Microbiology

Associate Professor

Hubertus Haas

phone: +43/512/9003-70205
email: hubertus.haas@i-med.ac.at

Group members

CV

 

Fungi affect the life of mankind positively and negatively. On the one hand, fungi are major players in saprobic decomposition, mutually interact with plants (mycorrhiza), serve directly as food (mushrooms) or in food production (e.g., bread, cheese, alcohol), and produce widely used primary (e.g. citrate, enzymes) and secondary metabolites (e.g. penicillin, cyclosporine A). On the other hand, some fungi are pathogens of plants (e.g. Fusarium spp.) and animals (e.g. Aspergillus fumigatus), or spoil food by contamination or toxin production (e.g. aflatoxin). Therefore, fungi impact ecology, biotechnology, medicine, agriculture and food industry. The best studied fungal organism is Saccharomyces cerevisiae. In some respects, however, the physiology of this yeast is not comparable with that of filamentous fungi (e.g. iron regulation and uptake, light regulation, secondary metabolism). We are mainly interested in the molecular elucidation of the peculiarities of filamemtous fungi«s physiology. 

Our current research focus is the iron/siderophore metabolism of A. fumigatus and A. nidulans. A. fumigatus is a typical saprobic filamentous ascomycete but also the most common airborne fungal pathogen of humans. It causes allergic and invasive disease depending on the immune system. Unsatisfying diagnostic and therapeutic possibilities are reflected in a high mortality rate. The low-pathogenic A. nidulans represents an established genetic model system. Both Aspergillus species produce an extracellular siderophore (triacetylfusarinine C) for iron acquisition and an intracellular siderophores (ferricrocin) for iron storage. Siderophore biosynthesis is regulated by two transcription factors, SreA and HapX. Siderophores are central components of the fungal metabolism as they affect germination, sexual and asexual reproduction, oxidative stress resistance and virulence. Lack of siderophore biosynthesis renders A. fumigatus apathogenic. Consequently, the siderophore system represents a novel target for antifungal therapy. 

Additional research topics are light regulation, nitrogen metabolism, noncoding RNA«s, improvement of molecular tools for the manipulation of fungi. Our research goal is to characterize the fungal metabolism and to exploit this knowledge for both improvement of antifungal therapy and improvement of the fungi«s biotechnological potential.

Haas7 Haas3 Haas4

 

Projects

Current Projects

Past Projects

 

Selected Publications

  1. Haas H, Redl B, Leitner E & Stöffler G (1991). Penicillium chrysogenum extracellular acid phosphatase: purification and biochemical characterization. Biochim. Biophys. Acta 1074:392-397.  

  2. Haas H, Redl B, Friedlin E & Stöffler G (1992). Isolation and molecular analysis of the Penicillium chrysogenum phoA gene encoding a secreted phosphate-repressible acid phosphatase. Gene 113:129-133.  

  3. Haas H, Redl B, Herfurth E & Stöffler G (1992). Purification, characterization and partial amino acid sequences of a xylanase produced by Penicillium chrysogenum. Biochim. Biophys. Acta 1117:279-286.  

  4. Haas H, Friedlin E, Stöffler G & Redl B (1993). Cloning and structural organization of a xylanase-encoding gene from Penicillium chrysogenumGene 126:237-242.  

  5. Haas H, Bauer B, Redl B, Stöffler G & Marzluf GA (1995). Molecular cloning and analysis of nre, the major nitrogen regulatory gene of Penicillium chrysogenumCurr. Genet. 27:150-158.  

  6. Haas H & Marzluf GA (1995). NRE, the major nitrogen regulatory protein of Penicillium chrysogenum, binds specifically in the intergenic promoter regions of nitrate assimilation and penicillin biosynthetic gene clusters. Curr. Genet. 28:177-183.  

  7. Marx F, Haas H, Hofer S, Stöffler G & Redl B (1995). Sequence and structure of Penicillium chrysogenum phoG, homologous to an acid phosphatase encoding gene of Aspergillus nidulansGene 160:137-138.  

  8. Marx F, Haas H, Reindl M, Stöffler G, Lottspeich F & Redl B (1995). Cloning, structural organization and regulation of expression of the Penicillium chrysogenum paf gene encoding an abundantly secreted protein with antifungal activity. Gene 167:167-171.  

  9. Haas H, Marx F, Graessle S & Stöffler G (1996). Sequence analysis and expression of the Penicillium chrysogenum nitrate reductase encoding gene (niaD). Biochim. Biophys. Acta 1309:81-84.  

  10. Haas H, Zadra I & Graessle S (1996). Partial sequence of the actin encoding gene (actA) of Penicillium chrysogenum. GenBank accession number U61733.  

  11. Haas H, Angermayr K & Stöffler G (1997). Molecular analysis of a Penicillium chrysogenum GATA factor encoding gene (sreP) exhibiting significant homology to the Ustilago maydis urbs1 gene. Gene 184:33-37.  

  12. Graessle S, Haas H, Friedlin E, Kürnsteiner H, Stöffler G & Redl B (1997). Regulated system for heterologous gene expression in Penicllium chrysogenum. Appl. Env. Microbiol. 63:753-756.  

  13. Lusser A, Brosch G, Loidl A, Haas H & Loidl P (1997). A Novel Type of Histonedeacetylases: Molecular Cloning and Sequencing of HD2 of Z. mays. 17th International Congress of Biochemistry and Molecular Biology, FASEB Journal 11(9). Abstract.  

  14. Lusser A, Brosch G, Loidl A, Haas H & Loidl P (1997). Identification of maize histone deacetylase HD2 as an acidic nucleolar phosphoprotein. Science 277:88-91.  

  15. Haas H, Angermayr K, Zadra I, & Stöffler G (1997). Overexpression of nreB, a new GATA factor-encoding gene of Penicillium chrysogenum, leads to repression of the nitrate assimilatory gene cluster. J. Biol. Chem. 272:22576-22582.  

  16. Zhou L, Haas H & Marzluf GA (1998). Isolation and characterization of a new gene, sre, which encodes a GATA-type regulatory protein that controls iron transport in Neurospora crassa. Mol. Gen. Genet. 259:532-540.  

  17. Dangl M, Lusser A, Brosch G, Loidl A, Haas H & Loidl P (1998). Second family of histone deacetylases. Science 280:1167a (www.sciencemag.org). Technical comment.  

  18. Angermayr K, Parson W, Stöffler G & Haas H (1999). Expression of atrC - encoding a novel member of the ATP-binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. Biochim. Biophys. Acta 1453:304-310.  

  19. Haas H, Zadra I, Stöffler G & Angermayr K (1999). The Aspergillus nidulans GATA-factor SREA is involved in regulation of siderophore biosynthesis and control of iron uptake. J. Biol. Chem. 274:4613-4619.  

  20. Haas H (1999). GATA- factors and nitrogen metabolism in filamentous fungi. Amino Acids 17:98. Abstract.  

  21. Lusser A, Eberharter A, Loidl A, Goralik-Schramel M, Haas H & Loidl P (1999). Analysis of the histone acetyltransferasae B complex of maize embryos. Nucl. Acids Res. 27:4427-35.  

  22. Graessle S, Dangl M, Haas H, Mair K, Trojer P, Brandtner E, Walton JD, Loidl P & Brosch G. (2000). Characterization of two putative histone deacylase genes from Aspergillus nidulans. Biochim. Biophys. Acta1492:120-6.  

  23. Feng B, Haas H & Marzluf GA (2000). ASD4, a new GATA factor of Neurospora crassa, displays sequence-specific DNA binding and functions in ascus and ascospore development. Biochemistry 39:11065-73.  

  24. Zadra I, Abt B, Parson W & Haas H (2000). AxylP promoter-based expression system and its use for antisense downregulation of the Penicillium chrysogenum nitrogen regulator NRE. Appl. Environ. Microbiol.66:4810-4816.  

  25. Oberegger H, Zadra I, Schoeser M & Haas H (2000). Iron starvation leads to increased expression of Cu/Zn-superoxide dismutase in Aspergillus. FEBS letters, 485:113-116.  

  26. Dangl M, Brosch G, Haas H, Loidl P & Lusser A (2001). Comparative analysis of HD2 type histone deacetylases in higher plants. Planta 213, 280-285.  

  27. Conlon H, Zadra I, Haas H, Arst HN, Jones MG & Caddick MX (2001). The Aspergillus nidulans GATA transcription factor gene areB encodes at least three proteins and features three classes of mutation. Mol. Microbiol. 40(2): 361-375.  

  28. Oberegger H, Schoeser M, Zadra I, Abt B & Haas H (2001). SREA is involved in regulation of siderophore biosynthesis, utilization and uptake in Aspergillus nidulans. Mol. Microbiol. 41(5): 1077-1089.  

  29. Oberegger H, Zadra I, Schoeser M, Abt B, Parson W & Haas H (2002). Identification of members of the Aspergillus nidulans SREA regulon: genes involved in siderophore biosynthesis and utilization. Bioch. Soc. Trans. 30(4):781-783  

  30. Oberegger H, Schoeser M, Zadra I, Schrettl M, Parson W & Haas H (2002). Regulation of freA, acoA, lysF,and cycA Expression by Iron Availability inAspergillus nidulans. Appl. Env. Microbiol. 68(11): 5769-5772.  

  31. Haas H, Schoeser M, Lesuisse E, Ernst JF, Parson W, Abt B, Winkelmann G &Oberegger H (2002). Characterisation of the Aspergillus nidulans transporters for the siderophores enterobactin and triacetylfusarinine C. Biochem J. 371:505-513. 

  32. Greene AV, Keller N, Haas H & Bell-Pedersen D (2003). A Circadian Oscillator in Aspergillus spp. Regulates Daily Development and Gene Expression. Eukaryot. Cell 2:231-237.

     Eisendle M, Oberegger H, Zadra I & Haas H (2003). The siderophor system is essential for viability of Aspergillus nidulans: functional analysis of two genes encoding L-ornithine N5-monooxygenase 8sidA) and a non-ribosomal peptide synthetase (sidC). Mol Microbiol 49:359-375. 

  33. Haas H (2003). Molecular genetics of fungal siderophore biosynthesis and uptake: the role of siderophores in iron uptake and storage. Appl. Microbiol. Biot. 62: 316-30  

  34. Trojer P, Brandtner E.M, Brosch G, Loidl P, Galehr J, Linzmaier R, Haas H, Mair K, Tribus M & Graessle S (2003). Histone deacetylases in fungi: novel members new facts. Nucl. Acids Res., 31, 3971-3981. 

  35. Oberparleiter, C., Kaiserer, L., Haas, H., Ladurner, P., Andratsch, M. and Marx, F.(2003). Active internalization of the Penicillium chrysogenum antifungal protein PAF in sensitive Aspergilli. Antimicrob. Agents Chemother 47: 3598-601.  

  36. Oberegger, H., Eisendle, M., Schrettl, M., Graessle, S. and Haas, H. (2003). 4'-phosphopantheinyl transferase encoding npgA is essential for siderophore biosynthesis in Aspergillus nidulans. Curr. Genet. 44: 211-5.  

  37. Eisendle, M., Oberegger, H., Buttinger, R., Illmer, P. and Haas, H. (2004) Biosynthesis and uptake of siderophores is controlled by the PacC-mediated ambient pH regulatory system in Aspergillus nidulans. Eukaryot. Cell 3: 561-3.  

  38. Leiter, E., Marx, F., Pusztahelyi, T., Haas, H. and Pocsi, I. (2004) Penicillium chrysogenum glucose oxidase - a study on its antifungal effects. J. Appl. Microbiol. 97: 1201-1209. 

  39. Schrettl, M., Winkelmann, G. and Haas, H. (2004) Ferrichrome in Schizosaccharomyces pombe - an iron transport and iron storage compound. Biometals 17: 647-54. 

  40. Nierman WC et al. (2005). Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature 438: 1151-1156. Erratum 2006 in Nature 439, 502. 

  41. Tribus, M., Galehr, J., Trojer, P., Brosch, G., Loidl, P., Marx, F., Haas, H., & Graessle, S. (2005). HdaA, a Major Class 2 Histone Deacetylase of Aspergillus nidulans Affects Growth Under Conditions of Oxidative Stress. Eukaryot. Cell 4, 1736-1745. 

  42. Fluckinger, M., Haas, H., Merschak, P., Glasgow, B.J. and Redl, B. (2004) Human Tear Lipocalin Exhibits Antimicrobial Activity by Scavenging Microbial Siderophores. Antimicrobial Agents Chemother 48: 3367-3372.

     Schrettl, M., Bignell, E., Kragl, C., Joechl, C., Rogers, T., Arst, H.N., Haynes, K. and Haas, H. (2004) Siderophore Biosynthesis but not Reductive Iron Assimilation is Essential for Aspergillus fumigatusVirulence. J. Exp. Med. 200: 1213-1219. 

  43. Oide, S., Moeder, W., Haas, H., Krasnoff, S., Gibson, D., Yoshioka, K. and Turgeon, G.S. (2006). NPS6, Encoding a Non-Ribosomal Peptide Synthetase Involved in Siderophore-Mediated Iron Metabolism, is a Conserved Virulence Determinant of Plant Pathogenic Ascomycetes. Plant Cell 18: 2836-53. 

  44. Eisendle, M., Schrettl, M., Kragl, C., Mueller, D., Illmer, P. and Haas, H. (2006). The intracellular siderophore ferricrocin is involved in iron storage, oxidative stress resistance, germination and sexual development in Aspergillus nidulans. Euk. Cell 5: 1596-603. 

  45. Hortschansky, P., Eisendle, M., Al-Abdallah, Q., Schmidt, A.D., Bergmann, S., Thoen, M., Kniemeyer, O., Abt, B., Seeber, B., Werner, E.R., Kato, M., Brakhage, A.A. and Haas, H. (2007). Interaction of HapX with the CCAAT-binding complex - a novel mechanism of gene regulation by iron. EMBO J. 26: 3157-68. 

  46. Kragl, C., Schrettl, M., Abt, B., Sarg, B., Lindner, H.H. and Haas, H. (2007). EstB-mediated hydrolysis of the siderophore triacetylfusarinine C optimizes iron uptake of Aspergillus fumigatus. Eukaryot. Cell 6: 1278-85. 

  47. Schrettl, M., Bignell, E., Kragl, C., Sabiha, Y., Loss, O., Eisendle, M., Wallner, A., Arst, H.N. Jr, Haynes, K. and Haas, H. (2007). Distinct Roles for Intra- and Extracellular Siderophores during Aspergillus fumigatus Infection. PLOS Pathogens 3: 1195-207. 

  48. Purschwitz J, Mueller S, Kastner C, Michelle Schoeser M, Haas H, Espeso EA, Atoui A, Calvo AM and Fischer R (2008). Functional and physical interaction of blue and red-light sensors in Aspergillus nidulans. Curr. Biol. 18:255-9.  

  49. Blum, G., Perkhofer, S., Haas, H., Schrettl, M., Wuerzner, R., Dierich, M.P., Lass-Floerl, C. (2008). Potential Basis for Amphotericin B Resistance in Aspergillus terreus. Antimicrob. Agents Chemother. 52:1553-5. 

  50. Haas, H., Eisendle, M., and Turgeon, G.S. (2008) Siderophores in fungal physiology and virulence. Annu. Rev. Phytopathol. 46:149-187. 

  51. Joechl, C., Rederstorff, M., Hertel, J., Stadler, P.F.,Hofacker, I., Schrettl, M., Haas, H., and Huettenhofer, A. (2008) Small ncRNA transcriptome analysis from Aspergillus fumigatus suggests a novel mechanism for regulation of protein-synthesis. Nucl. Acids Res. 36:2677-89. 

  52. Schrettl M, Kim HS, Eisendle M, Kragl C, Nierman WC, Heinekamp T, Werner ER, Jacobsen I, Illmer P, Yi H, Brakhage AA & Haas H (2008). SreA-mediated iron regulation in Aspergillus fumigatus. Mol Microbiol70:27-43. 

  53. McDonagh A, Fedorova ND, Crabtree J, Yu Y, Kim S, Chen D, Loss O, Cairns T, Goldman G, Armstrong-James D, Haynes K, Haas H, Schrettl M, May G, Nierman WC & Bignell E (2008). Sub-telomere directed gene expression during initiation of invasive aspergillosis. PLoS Pathog 4(9):e1000154. 

  54. Seifert M, Nairz M, Schroll A, Schrettl M, Haas H & Weiss G (2008). Effects of the Aspergillus fumigatus siderophore systems on the regulation of macrophage immune effector pathways and iron homeostasis. Immunobiology 213:767-778 

  55. Jöchl C, Loh E, Ploner A, Haas H & Hüttenhofer A (2009). Development-dependent scavenging of nucleic acids in the filamentous fungus Aspergillus fumigatus. RNA biology 6, :179-186. 

  56. Wallner A, Blatzer M, Schrettl M, Sarg B, Lindner H & Haas H (2009). Ferricrocin - a siderophore involved in intra- and transcellular iron distribution in Aspergillus fumigatus. Appl Environ Microbiol 75:4194-4196. 

  57. Sabiha Y, Abt B, Schrettl M, Moussa-TAA & Haas H (2009). The interplay between iron and zinc metabolism in Aspergillus fumigatus. Fungal Genet Biol 46:707-13 

  58. Chamilos G, Bignell E, Schrettl M, Lewis RE, Leventakos K, May GS, Haas H & Kontoyiannis DP (2010). Exploring the Concordance of Aspergillus fumigatus Pathogenicity in Mice and Toll-Deficient Flies. Med Mycol 47:1-5.  

  59. Thön M, Al-Abdallah Q Hortschanski P, Scharf DH, Eisendle M, Haas H & Brakhage AA (2010). The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes. Nucl Acids Res 38:1098-1113. 

  60. Tribus M, Bauer I, Haas H, Brosch G. & Graessle S. (2010). A conserved motif in fungal class 1 histone deacetylases is essential for growth and development of Aspergillus nidulans. Mol Biol Cell 21:345-353.  

  61. Petrik M, Haas H, Dobrozemsky G, Lass-Flšrl C, Helbok A, Dietrich H & Decristoforo C (2010) 68Ga-siderophores for PET imaging of invasive pulmonary aspergillosis - proof of principle. J Nucl. Med. 51:639-45. 

  62. Schrettl M, Carberry S, Kavanagh K, Haas H, Nolan A & Doyle S. (2010). Self-protection against gliotoxin - a component of the gliotoxin biosynthetic cluster, GliT, completely protects Aspergillus fumigatus against exogenous gliotoxin. PLOS Pathogens 10;6(6):e1000952. 

  63. Schrettl M, Ibrahim-Granet O, Droin S, Huerre M, Latge JP & Haas H (2010). The crucial role of siderophores in interaction of Aspergillus fumigatus in interaction with alveolar macrophages. Microbes and Infection 12:1035-41. 

  64. Schrettl M, Beckmann N, Varga J, Heinekamp T, Jacobsen ID, Joechl C, Moussa TA, Wang S, Gsaller F, Blatzer M, Werner ER, Niermann W, Brakhage AA & Haas H (2010). HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus. PLOS Pathogens 30;6(9). pii: e1001124. 

  65. Sigl C, Haas H, Pfaller K, Kuernsteiner H & Ivo Zadra (2011). Among developmental regulators StuA but not BrlA is essential for Penicillin V production in Penicillium chrysogenum. Appl Env Microbiol 77:972-82. 

  66. Voedisch M, Scherlach K, Winkler R, Hertweck C, Braun HP, Roth M, Haas H, Werner ER, Brakhage AA & Kniemeyer O (2011). Analysis of the Aspergillus fumigatus proteome reveals metabolic changes and the activation of the pseurotin A biosynthesis gene cluster in response to hypoxia. J Proteome Res 10:2508-24. 

  67. Blatzer M, Schrettl M, Sarg B, Lindner H, Pfaller K & Haas H (2011). SidL, an A. fumigatus transacetylase involved in biosynthesis of the siderophores ferricrocin and hydroxyferricrocin. Appl Env Microbiol 77:4959-6. 

  68. Jain R, Valiante V, Remme N, Docimo T, Heinekamp T, Hertweck C, Gershenzon J, Haas H, Brakhage AA (2011). The MAP Kinase MpkA controls cell wall integrity, oxidative stress response and iron adaptation in A. fumigatus. Mol Microbiol 82:39-53

  69. Blatzer M, Binder U & Haas H (2011). The metalloreductase FreB is involved in adaptation of A. fumigatus to iron starvation. Fungal Genet Biol 48:1027-1033. 

  70. Blatzer M, Barker BM, Willger SD, Beckmann N, Blosser SJ, Cornish EJ, Mazurie A, Grahl N, N, Haas & H Cramer R (2011). SREBP Coordinates Iron and Ergosterol Homeostatis to Mediate Triazole Drug and Hypoxia Responses in the Human Fungal Pathogen Aspergillus fumigatus. PLoS Genetics 7(12):e1002374.

  71. Hegedues N, Leiter E, Kovacs B, Tomori V, Kwon NJ, Emri T, Marx F, Batta G, Csernoch L, Haas H, Yu JH & P—csi I (2011). The small molecular mass antifungal protein of Penicillium chrysogenum - a mechanism of action oriented review. J Basic Microbiol 51:561-571. 

  72. Schrettl M & Haas H (2011). Iron homeostasis - Achilles' heel of A. fumigatus? Curr Oppin Microbiol 14:400-5. 

  73. Yasmin S, Alcazar-Fuoli L, Gründlinger M, Puempel T, Cairns T, Lopez JF, Grimalt JO, Blatzer M, Bignell E & Haas H (2012). Mevalonate Governs Interdependency of Ergosterol and Siderophore Biosyntheses in the Fungal Pathogen Aspergillus fumigatus. PNAS 109(8):E497-504. 

  74. Petrik M, Haas H, Schrettl M, Helbok A, Blatzer M & Decristoforo C (2012). In vitro and in vivo evaluation of selected 68Ga-siderophores for infection imaging. Nucl Med Biol 39:361-9.  

  75. Linde J, Hortschansky P, Facius E, Brakhage AA, Guthke R & Haas H (2012). Regulatory interactions for iron homeostasis in Aspergillus fumigatus inferred from application of the systems biology circle. BMC Systems Biol 6:6 

  76. Petrik M, Haas H, Schrettl M, Blatzer M & Decristoforo C (2012). Preclinical evaluation of two 68Ga-siderophores as potential radiopharmaceuticals for Aspergillus fumigatus infection imaging. Eur J Nucl Med Mol Imaging 39:1175-83 

  77. Lopez-Berges M S, Capilla J, Turra D, Schafferer L, Matthijs S, Joechl C, Cornelis P, Guarro J, Haas H, Di Pietroa A (2012). HapX-Mediated Iron Homeostasis Is Essential for Rhizosphere Competence and Virulence of the Soilborne Pathogen Fusarium oxysporum. The Plant Cell 24(9):3805-22. 

  78. Gsaller F, Eisendle M, Lechner B, Schrettl, D, Sarg B, Lindner H, M Mueller, Geley S & Haas H (2012). The interplay of vacuolar and siderophore-mediated iron storage in A. fumigatus resistance. Metallomics, 4:1262-70  

  79. Haas H (2012). Iron - a key nexus in the virulence of Aspergillus fumigtus. Front Microbiol, 3:28. 

  80. Chung D, Haas H & Cramer R (2012). Coordination of hypoxia adaptation and iron homeostasis in pathogenic fungi. Front Microbiol, 3:381. 

  81. Gsaller F, Blatzer M, Schrettl M, Lindner H, & Haas H (2013). The first promoter for conditional gene expression in Acremonium chrysogenum: iron starvation-inducible mir1P. J Biotechnol, 163:77-80. 

  82. López-Berges MS, Turrá D, Capilla J, Schafferer L, Matthijs S, Jöchl C, Cornelis P, Guarro J, Haas H & Di Pietro A (2013). Iron competition in fungus-plant interactions: The battle takes place in the rhizosphere. Plant Signal & Behav, 8(2). doi:pii: e23012. 

  83. Gründlinger M, Yasmin S, Lechner B, Geley S, Schrettl M, Hynes M & and Haas H (2013). Fungal siderophore biosynthesis is partially localized in peroxisomes. Mol Microbiol, 88:862-75. 

  84. Leal SM., Roy S., Vareechon C., Clark H., Carrion S., Lopes-Berges MS., di Pietro A., Schrettl M., Beckman N., Redl B., Haas H. and Pearlman E. (2013) Targeting iron acquisition blocks infection with the fungal pathogens Aspergillus fumigatus and Fusarium oxysporum. PLOS Pathogens, 9(7):e1003436 

  85. Beckmann N, Schafferer L, Schrettl M, Binder U, Lindner H, Haas H. (2013) Characterization of the link of ornithine, arginine, polyamine and siderophore metabolism in Aspergillus fumigatus. PLoS One , 18;8(6):e67426. 

  86. Amich J, Schafferer L, Haas H & Krappmann S (2013). Regulation of sulphur assimilation is essential for virulence and impacts iron homeostasis of the human-pathogenic mould Aspergillus fumigatus. PLoS Pathogens, 9(8):e1003573. 

  87. Gründlinger M, Schrettl M, Gsaller F, Lindner H & and Haas H (2013). Aspergillus fumigatus SidJ mediates siderophore hydrolysis. Appl Env Microbiol, 88(5):862-75. 

  88. Steinchen W, Lackner G, Yasmin S, Schrettl M, Dahse HM, Haas H, Hoffmeister D (2013). The bimodular peptide synthetase SidE produces fumarylalanine in the human pathogen Aspergillus fumigatus. Appl Env Microbiol , 79(21):6670-6.  

  89. Franken ACW, Werner ER, Haas H, Lokman BC, Ram AFJ, van den Hondel CAMJJ, de Weert S & Punt PJ (2013). The role of Coproporphyrinogen III oxidase and Ferrochelatase genes in heme biosynthesis and regulation in Aspergillus niger. Appl Microbiol Biot, 97:9773-85. 

  90. Haselwandter K, Häninger G, Ganzera M, Haas H, Nicholson G, Winkelmann G (2013). Linear fusigen as the major hydroxamate siderophore of the ectomycorrhizal Basidiomycota Laccaria laccata and Laccaria bicolor. Biometals , 26:969-79. 

  91. Petrik M, Haas H, Schrettl M, Blatzer M & Decristoforo C (2014). 68Ga-Triacetylfusarinine C and 68Ga-Ferrioxamine E for Aspergillus infection imaging: uptake specificivity testing in various microorganisms. Mol Imaging Biol, 16(1):102-8. 

  92. Blatzer M, Gsaller F, Abt B, Schrettl M, & Haas H (2014). An endogenous promoter for conditional gene expression in Acremonium chrysogenum: the xylan and xylose inducible promoter xyl1P. J Biotechnol, 169:82-86. 

  93. Albarouki E, Schafferer L, Ye F, von Wirén N, Haas H, Deising HB (2014). Biotrophy-specific down-regulation of siderophore biosynthesis in Colletotrichum graminicola is required for modulation of immune responses of maize. Mol Microbiol, 92:338-55. 

  94. Vergeiner S, Schafferer L, Haas H & Müller T (2014). Improved MALDI-TOF Microbial Mass Spectrometry Imaging by Application of a Dispersed Solid Matrix. J Am Soc Mass Spectrom, 25:1498-501. 

  95. Hillmann F, Linde J, Beckmann N, Cyrulies M, Strassburger M, Haas H, Guthke R, Kniemeyer O & Brakhage AA (2014). The novel sensor-globin fungoglobin is involved in low oxygen adaptation of Aspergillus fumigatus. Mol Microbiol, 93(3):539-53. doi: 10.1111. 

  96. Gsaller F, Hortschansky P, Beattie SR, Klammer K, Tuppatsch K, Lechner BE, Rietzschel N, Werner ER, Vogan AA, Chung D, Mühlenhoff U, Kato M, Cramer RA, Brakhage AA & Haas H (2014). HapX is a Janus-type transcription factor. EMBO J, 33:2261-76. doi: 10.15252/embj.201489468. 

  97. Franken ACW, Werner ER, Haas H, Lokman BC, van den Hondel CAMJJ, Ram AFJ, de Weert S & Punt PJ (2014). Genome mining and functional genomics for siderophore production in Aspergillus niger. Brief Funct Genom, 13:482-92. doi: 10.1093/bfgp/elu026. 

  98. Silva MG1, Bailão LC, Lechner BE, Gauthier GM, Lindner H, Bailão AM, Haas H & Soares CM (2014). Hydroxamate production as a high affinity iron acquisition mechanism in Paracoccidioides spp. PLoS One, 9(8):e105805. 

  99. Haas H (2014). Fungal siderophore metabolism with a focus on Aspergillus fumigatus. Natural Product Reports, 31(10):1266-76. 

  100. Bertuzzi M, Schrettl M, Alcazar-Fuoli L, Cairns TC, Munoz A, Walker L, Herbst S, Safari M, Cheverton A, Chen D, Liu H, Saijo S, Fedorova ND, Armstrong-James D, Munro C, Read ND, Filler SG, Espeso EA, Nierman WC, Haas H & Bignell EM (2014). The A. fumigatus pH-responsive PacC transcription factor governs epithelial invasion during pulmonary aspergillosis. PLoS Pathogens, 10(10):e1004413. doi: 10.1371 (MS is co-1st author). 

  101. Wiemann P, Lechner BE, Baccile JA, Velk TA, Yin WB, Bok JW, Pakala S Losada L, Nierman WC, Schroeder FC, Haas H & Keller NP (2014). Perturbations in small molecule synthesis uncovers an iron-responsive secondary metabolite network in Aspergillus fumigatus. Frontiers Microbiol, 5:530. doi: 10.3389. 

  102. Chung D, Barker BM, Carey CC, Merriman B, Werner ER, Lechner BE, Dhingra S, Cheng C, Xu W, Blosser SJ, Morohashi K, Mazurie A, Mitchell TK, Haas H, Mitchell AP, and Cramer RA (2014). ChIP-seq and in vivo transcriptome analyses of the Aspergillus fumigatus SREBP SrbA reveals new insights into fungal hypoxia adaptation and virulence. PLoS Pathogens, 10(11):e1004487. 

  103. Petrik M, Vlckova A, Novy Z, Haas H & Decristoforo C (2014). Selected 68Ga-siderophores versus 68Ga-Colloid and 68Ga-citrate: biodistribution and small animal imaging in mice. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, doi: 10.5507, in press. 

  104. Knetsch PA, Chuangyan Z, Rangger C, Blatzer M, Haas H, Kaeopookum P, Haubner R & Decristoforo C (2014). [68Ga]FSC-(RGD)3, a trimeric RGD peptide for imaging αvβ3 integrin expression based on a novel siderophore derived chelating scaffold - synthesis and evaluation. Nucl Med Biol, 42:115-22. doi: 10.1016/j.nucmedbio.2014.10.001. 

  105. Briard B, Bomme P, Lechner BE, Mislin GLA, Lair V, Prévost MC, Latgé JP, Haas H & Beauvais A (2015). In Aspergillus fumigatus, phenazines target redox and iron homeostasis depending on the derivative. Sci Rep, in press. 

  106. Hortschansky P, Ando E, Tuppatsch K, Arikawa T, Kobayashi T, Kato M, Haas H & Brakhage AA (2015) Deciphering the DNA-binding code of the multicomponent iron-regulatory transcription factor complex CBC:HapX. J Biol Chem, in press. 

  107. Haas H, Petrik M & Decristoforo (2015). An iron-mimicking Trojan horse as fungi's Achilles heel - has the time come for molecular imaging of fungal infections? PLoS Pathogens, in press.

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