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Protocols

On this page I will publish protocols for microscopy in pdf format.

Cell-IQ

• Scratch assay (Lukas Peintner)

SP5 confocal microscope

Highly sensitive hybrid detectors (HyDs, 01/2013)

• General Info
• Large dynamic range
• Number&Brightness Analysis (recommended for advanced users only)

• Other protocols

• Objective_correction : Summary how to optimize the objectives using the correction collars!
• SP5 image data: How to extraxt image infos from lif files
• SP5_errorLog: How to generate an error report on the SP5 microscope
• Perfusion system: How to add substances, replace medium, without "on scope pipetting"!
• Leica protocols for the SP5 microscope:
• Dye separation: How to seperate dyes with a strong fluorescence overlap-linear unmixing.
• FRAP: How to perform FRAP and photoactivation experiments.
• FRET:FRET acceptor bleaching and FRET sensitized emission wizards.
• Live data mode: usage of the "live data mode" to combine different time series, multiposition imaging,..."programming" the SP5 scope.
• Glycerol objective:information on the extra-wide working distance glycerol objective-recommended for thick samples such as brain sections,...

• Huygens Deconvolution (& SP5)

• SP5_deconvolution: Instructions how to aquire images for deconvolution using the Huygens software.
• Huygens UserGuide
• How to get a measured PSF.
• Advantages of water immersion in live cell imaging