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Stefan Grässle, Ph.D., Associate Professor

EMail: stefan.graessle@i-med.ac.at

Phone: +43/(0)512/9003-70218

Fax: +43/(0)512-9003-73100

Short curriculum

Research fields

• Functional roles of histone modifications in filamentous fungi
• Penicillium chrysogenum as an expressions system for homologous und heterologous gene expression
• Production of immunodominant proteins of Helicobacter pylori and Borrelia burgdorferi in prokaryotic expression systems
• Histone deacetylation and gene regulation in filamentous fungi

Current research project

• Histone Deacetylases in Fungi: Novel Functions (supported by the Austrian Science Foundation (FWF), Project P-24803)

Teaching

From the online catalog of the Innsbruck Medical University (in German): Current lectures

From the online catalog of the University of Innsbruck (in German): Current lectures

Publications

1. Karahoda, B., Pardeshi, L., Ulas, M., Dong, Z., Shirgaonkar, N., Guo, S., Wang, F., Tan, K., Sarikaya-Bayram, Ö., Bauer, I., Dowling, P., Fleming, A.B., Pfannenstiel, B.T., Luciano-Rosario, D., Berger, H., Graessle, S., Alhussain, M.M., Strauss, J., Keller, N.P., Wong, K.H., Bayram, Ö. (2022). The KdmB-EcoA-RpdA-SntB chromatin complex binds regulatory genes and coordinates fungal development with mycotoxin synthesis. Nucleic Acids Res. doi: 10.1093/nar/gkac744
2. Bauer, I. and Graessle, S. (2021). Fungal Lysine Deacetylases in Virulence, Resistance, and Production of Small Bioactive Compounds. Genes 12,1470. doi: 10.3390/genes12101470
3. Bauer, I., Gross S., Merschak, P., Kremser, L., Karahoda, B., Sarikaya Bayram, Ö., Abt, B., Binder, U., Gsaller, F., Lindner, H., Bayram, Ö., Brosch, G. and Graessle, S. (2020) RcLS2F – A Novel Fungal Class 1 KDAC Co-repressor Complex in Aspergillus nidulans. Front. Microbiol. 11:43. doi: 10.3389/fmicb.2020.00043
4. Bauer, I., Misslinger, M., Shadkchan, Y., Dietl, A.M., Petzer, V., Orasch, T., Abt, B.,  Graessle, S., Osherov, N. and Haas, H. (2019). The Lysine Deacetylase RpdA Is Essential for Virulence in Aspergillus fumigatus. Front. Microbiol 10:2773
5. Bauer, I., Lechner, L., Pidroni, A., Petrone, A., Merschak, P., Lindner, H., Kremser, L., Graessle, S., Golderer, G., Allipour, S. and Brosch, G. (2019). Type I and II PRMTs regulate catabolic as well as detoxifying processes in Aspergillus nidulans. Fungal Genet and Biol. 129, 86-100. doi: 10.1016/j.fgb.2019.05.006
6. Bauer, I., Pidroni, A., Bayram, Ö., Brosch, G., Graessle, S. (2019). Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay. J. Vis. Exp. 147: e59527. doi: 10.3791/59527.
7. Pidroni A., Faber B., Brosch G., Bauer I. and Graessle S. (2018). A Class 1 Histone Deacetylase as Major Regulator of Secondary Metabolite Production in Aspergillus nidulans. Front. Microbiol. 9:2212. doi: 10.3389/fmicb.2018.02212
8. Bauer, I., Varadarajan, D., Pidroni, A., Gross, S., Vergeiner, S., Faber, B., Hermann, M., Tribus, M., Brosch, G., Graessle, S. (2016). A Class 1 Histone Deacetylase with Potential as an Antifungal Target. mBio vol. 7 no. 6 e00831-16. doi: 10.1128/mBio.00831-16
9. Bauer, I., Graessle, S., Loidl, P., Hohenstein, K. and Brosch, G. (2010). Novel insights into the functional role of three protein arginine methyltransferases in Aspergillus nidulans. Fungal Genet Biol 47(6):551-61. 
10. Tribus, M., Bauer, I., Galehr, J., Rieser, G., Brosch, G., Loidl, P., Trojer, P., Haas, H. and Graessle, S. (2010). A novel motif in fungal class 1 histone deacetylases is essential for growth and development of Aspergillus. Molecular Biology of the Cell Vol. 21, 345.
11. Brosch, G., Loidl, P. & Graessle, S. (2008). Histone Modifications and Chromatin Dynamics: A Focus on Filamentous Fungi. FEMS Microbiol. Rev. 32, 409.
12. Shwab, E.K., Bok, JW., Tribus, M., Galehr, J., Graessle, S. and Keller, N. (2007). Histone deacetylase activity regulates chemical diversity in Aspergillus nidulans. Euk. Cell 9, 1656.
13. Tribus, M., Galehr, J., Trojer, P., Brosch, G., Loidl, P., Marx, F., Haas, H., & Graessle, S. (2005). HdaA, a Major Class 2 Histone Deacetylase of Aspergillus nidulans Affects Growth Under Conditions of Oxidative Stress. Euk. Cell 4, 1736.
14. Marx, F., Salvenmoser, W., Kaiserer, L., Graessle, S., Weiler-Gorz, R., Zadra, I. and Oberparleiter, C. (2005). Proper folding of the antifungal protein PAF is required for optimal activity. Res. Microbiol. 156, 35.  
15. Trojer, P., Dangl, M., Bauer, I., Graessle, S., Loidl, P. & Brosch, G. (2004). Histone methyltransferases in Aspergillus nidulans: evidence for a novel enzyme with a unique substrate specificity. Biochemistry 43, 10834.
16. Oberegger, H., Eisendle, M., Schrettl, M., Graessle, S. and Haas, H. (2003). 4'-phosphopantheinyl transferase encoding npgA is essential for siderophore biosynthesis in Aspergillus nidulans. Curr. Genet. 44, 211.
17. Trojer, P., Brandtner, E.M., Brosch, G., Loidl, P., Galehr, J., Linzmaier, R., Haas, H., Mair, K., Tribus, M. and Graessle, S. (2003). Histone deacetylases in fungi: novel members new facts. Nucl. Acids Res. 31, 3971.
18. Baidyaroy, D., Brosch, G., Graessle,S., Trojer, P. and Walton, J.D. (2002). Characterization of inhibitor-resistant histone deacetylase activity in plant-pathogenic fungi. Euk. Cell 1, 538.
19. Brosch, G., Dangl, M., Graessle, S., Trojer, P., Brandtner, E.M., Mair, K., Walton, J.D., Baidyaroy, D. and Loidl P. (2001). An inhibitor-resistant histone deacetylase in the plant pathogenic fungus Cochliobolus carbonum. Biochemistry 40, 12845.
20. Brosch, G., Baidyaroy, D., Ahn, J., Graessle, S., Wegener, S., Tonukari, N.J., Caballero, O., Loidl, P. and Walton, J.D. (2001). A gene related to yeast HOS2 histone deacetylase affects extracellular depolymerase expression and virulence in a plant pathogenic fungus. Plant Cell 13, 1609.
21. Graessle, S., Loidl, P. and Brosch, G. (2001) Histone acetylation: plants and fungi as model systems for the investigation of histone deacetylases. Cell. Mol. Life Sci 58, 704.
22. Graessle, S., Dangl, M., Haas, H., Mair, K., Trojer, P., Brandtner E.M., Walton, J., Loidl, P. and Brosch, G. (2000). Characterization of two putative histone deacetylase genes from Aspergillus nidulans. Biochim. Biophys. Acta 1492, 120.
23. Graessle, S., Grabher, G., Gapp, G., Preuss, E., Datz, C., Sandhofer, F. and Stöffler, G. (2000). Immune response to natural and recombinant antigens of Helicobacter pylori in patients with dyspeptic complaints. Clin. Lab. 46, 220.
24. Graessle, S., Grabher, G., Gapp, G., Preuss, E., Datz, C., Sandhofer, F. and Stöffler, G. (1999). Immune response to natural and recombinant antigens of Helicobacter pylori in patients with dyspeptic complaints. Eur. J. Clin. Microbiol. Infect. Dis. 18, 636.
25. Graessle, S., Preuss, E., Grabher, G., Datz, C., Sandhofer, F. and Stöffler, G. (1997) Immune response to Helicobacter pylori as studied with natural and recombinant antigens. Clin. Microbiol. Infect. 3, Supplement 2: 226.
26. Graessle, S., Haas, H., Friedlin, E., Kürnsteiner, H., Stöffler, G. and Redl, B. (1997) Regulated system for heterologous gene expression in Penicillium chrysogenum. Appl. Environ. Microbiol. 63, 753.
27. Haas, H., Marx, F., Graessle, S. and Stöffler, G. (1996). Sequence analysis and expression of the Penicillium chrysogenum nitrate reductase encoding gene (niaD). Biochim. Biophys. Acta 1309, 81.