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Molecular Membrane Physiology & Functional Proteomics

The main interest of the lab is the characterization of proteins and protein networks that regulate the physiology of cells via the cell membrane. The cell membrane is semi-permeable and constitutes a barrier between the cell interior and the surrounding environment. To a great part, cellular homoeostasis is therefore maintained through the action of membrane proteins that accomplish a wide array of functions such as the generation of the resting membrane potential; maintenance of intracellular pH, ionic strength and hydration; adhesion; secretion; solute uptake; and last but not least, signal uptake and transduction into and out of the cell.

Within this field of research we are focusing on the physiology of the pancreatic islet of Langerhans and the regulation of insulin secretion.

Methods employed in the lab include live cell imaging (intracellular calcium and pH measurements, protein/protein interactions by FRET, exocytosis), protein biochemistry as well as molecular biology. 

 

Latest Publications

 

  1. 2017: The H(+)/K(+)-ATPase Inhibitor SCH-28080 Inhibits Insulin Secretion and Induces Cell Death in INS-1E Rat Insulinoma Cells. Jakab, M., Fürst J., Beyreis M., Kittl M., Kiesslich T., Hauser-Kronberger C., Gaisberger M., Ritter M. Cell Physiol Biochem 43, 1037-1051 https://www.ncbi.nlm.nih.gov/pubmed/28968600
  2. 2014: The putative role of the non-gastric H(+)/K(+)-ATPase ATP12A (ATP1AL1) as anti-apoptotic ion transporter: effect of the H(+)/K(+) ATPase inhibitor SCH28080 on butyrate-stimulated myelomonocytic HL-60 cells. Jakab, M., Hofer, S., Ravasio, A., Huber, F., Schmidt, S., Hitzl, W., Geibel, J.P., Furst, J., and Ritter, M. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 34, 1507-1526 http://www.ncbi.nlm.nih.gov/pubmed/25322912
  3. 2012: Effect of the AMP-kinase modulators AICAR, metformin and compound C on insulin secretion of INS-1E rat insulinoma cells under standard cell culture conditions, Langelueddecke, C., Jakab, M., Ketterl, N., Lehner, L., Hufnagl, C., Schmidt, S., Geibel, J.P., Fuerst, J., and Ritter, M. Cell Physiol Biochem 29, 75-86 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22415077
  4. 2011: The C-terminus of ICln is natively disordered but displays local structural preformation, Schedlbauer, A., Gandini, R., Kontaxis, G., Paulmichl, M., Furst, J., and Konrat, R. Cell Physiol Biochem 28, 1203-1210 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22179008
  5. 2011: The molecular and functional interaction between ICln and HSPC038 proteins modulates the regulation of cell volume, Dossena, S., Gandini, R., Tamma, G., Vezzoli, V., Nofziger, C., Tamplenizza, M., Salvioni, E., Bernardinelli, E., Meyer, G., Valenti, G., Wolf-Watz, M., Furst, J., and Paulmichl, M. J Biol Chem 286, 40659-40670 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21917931
  6. 2009: The phytostilbene resveratrol induces apoptosis in INS-1E rat insulinoma cells, Bortolotti, C., Kunit, T., Moder, A., Hufnagl, C., Schmidt, S., Hartl, A., Langelueddecke, C., Furst, J., Geibel, J.P., Ritter, M., and Jakab, M. Cell Physiol Biochem 23, 245-254 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19471092
  7. 2009: Quaternary structure assessment of ICln by fluorescence resonance energy transfer (FRET) in vivo, Schmidt, S., Jakab, M., Costa, I., Furst, J., Ravasio, A., Paulmichl, M., Botta, G., and Ritter, M. Cell Physiol Biochem 23, 397-406 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19471107
  8. 2008: Fixation, mounting and sealing with nail polish of cell specimens lead to incorrect FRET measurements using acceptor photobleaching, Rodighiero, S., Bazzini, C., Ritter, M., Furst, J., Botta, G., Meyer, G., and Paulmichl, M. Cell Physiol Biochem 21, 489-498 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18453757
  9. 2008: LSm4 associates with the plasma membrane and acts as a co-factor in cell volume regulation, Gandini, R., Dossena, S., Vezzoli, V., Tamplenizza, M., Salvioni, E., Ritter, M., Paulmichl, M., and Furst, J. Cell Physiol Biochem 22, 579-590 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19088440
  10. 2006a: Fast fluorometric method for measuring pendrin (SLC26A4) Cl-/I- transport activity, Dossena, S., Rodighiero, S., Vezzoli, V., Bazzini, C., Sironi, C., Meyer, G., Furst, J., Ritter, M., Garavaglia, M.L., Fugazzola, L., Persani, L., Zorowka, P., Storelli, C., Beck-Peccoz, P., Botta, G., and Paulmichl, M. Cell Physiol Biochem 18, 67-74 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16914891
  11. 2006b: Functional characterization of wild-type and a mutated form of SLC26A4 identified in a patient with Pendred syndrome, Dossena, S., Vezzoli, V., Cerutti, N., Bazzini, C., Tosco, M., Sironi, C., Rodighiero, S., Meyer, G., Fascio, U., Furst, J., Ritter, M., Fugazzola, L., Persani, L., Zorowka, P., Storelli, C., Beck-Peccoz, P., Botta, G., and Paulmichl, M. Cell Physiol Biochem 17, 245-256 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16791000
  12. 2006: Glucose induces anion conductance and cytosol-to-membrane transposition of ICln in INS-1E rat insulinoma cells, Jakab, M., Grundbichler, M., Benicky, J., Ravasio, A., Chwatal, S., Schmidt, S., Strbak, V., Furst, J., Paulmichl, M., and Ritter, M. Cell Physiol Biochem 18, 21-34 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16914887
  13. 2006: The ICln interactome, Furst, J., Botta, G., Saino, S., Dopinto, S., Gandini, R., Dossena, S., Vezzoli, V., Rodighiero, S., Bazzini, C., Garavaglia, M.L., Meyer, G., Jakab, M., Ritter, M., Wappl-Kornherr, E., and Paulmichl, M. Acta Physiol (Oxf) 187, 43-49 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16734741
  14. 2005: The expression of wild-type pendrin (SLC26A4) in human embryonic kidney (HEK 293 Phoenix) cells leads to the activation of cationic currents, Dossena, S., Maccagni, A., Vezzoli, V., Bazzini, C., Garavaglia, M.L., Meyer, G., Furst, J., Ritter, M., Fugazzola, L., Persani, L., Zorowka, P., Storelli, C., Beck-Peccoz, P., Botta, G., and Paulmichl, M. Eur J Endocrinol 153, 693-699 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16260428
  15. 2005: ICln159 folds into a pleckstrin homology domain-like structure. Interaction with kinases and the splicing factor LSm4, Furst, J., Schedlbauer, A., Gandini, R., Garavaglia, M.L., Saino, S., Gschwentner, M., Sarg, B., Lindner, H., Jakab, M., Ritter, M., Bazzini, C., Botta, G., Meyer, G., Kontaxis, G., Tilly, B.C., Konrat, R., and Paulmichl, M. J Biol Chem 280, 31276-31282 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15905169
  16. 2005: Thiazide-sensitive NaCl-cotransporter in the intestine: possible role of hydrochlorothiazide in the intestinal Ca2+ uptake, Bazzini, C., Vezzoli, V., Sironi, C., Dossena, S., Ravasio, A., De Biasi, S., Garavaglia, M., Rodighiero, S., Meyer, G., Fascio, U., Furst, J., Ritter, M., Botta, G., and Paulmichl, M. J Biol Chem 280, 19902-19910 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15781471
  17. 2004: Further characterization of the nematode IClnN2 protein reconstituted in lipid bilayers, Ritter, M., Bertocchi, C., Jakab, M., Furst, J., and Paulmichl, M. Adv Exp Med Biol 559, 245-251 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18727245
  18. 2004a: Ito channels are octomeric complexes with four subunits of each Kv4.2 and K+ channel-interacting protein 2, Kim, L.A., Furst, J., Butler, M.H., Xu, S., Grigorieff, N., and Goldstein, S.A. J Biol Chem 279, 5549-5554 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14623880
  19. 2004: Membrane thickness changes ion-selectivity of channel-proteins, Garavaglia, M., Dopinto, S., Ritter, M., Furst, J., Saino, S., Guizzardi, F., Jakab, M., Bazzini, C., Vezzoli, V., Dossena, S., Rodighiero, S., Sironi, C., Botta, G., Meyer, G., Henderson, R.M., and Paulmichl, M. Cell Physiol Biochem 14, 231-240 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15319526
  20. 2004: A new gene-finding tool: using the Caenorhabditis elegans operons for identifying functional partner proteins in human cells, Eichmuller, S., Vezzoli, V., Bazzini, C., Ritter, M., Furst, J., Jakab, M., Ravasio, A., Chwatal, S., Dossena, S., Botta, G., Meyer, G., Maier, B., Valenti, G., Lang, F., and Paulmichl, M. J Biol Chem 279, 7136-7146 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14615488
  21. 2004: Probing of the ICln channel pore by cysteine mutagenesis and cadmium-block, Jakab, M., Garavaglia, M.L., Furst, J., Rodighiero, S., Guizzardi, F., Meyer, G., Ritter, M., and Paulmichl, M. Adv Exp Med Biol 559, 99-108 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18727231
  22. 2004b: Three-dimensional structure of I(to); Kv4.2-KChIP2 ion channels by electron microscopy at 21 Angstrom resolution, Kim, L.A., Furst, J., Gutierrez, D., Butler, M.H., Xu, S., Goldstein, S.A., and Grigorieff, N. Neuron 41, 513-519 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14980201

Johannes Fuerst