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  • El Ghaleb Y, Campiglio M, Flucher BE. (2019) Correcting the R165K substitution in the first voltage-sensor of CaV1.1 right-shifts the voltage-dependence of skeletal muscle calcium channel activation. Channels (Austin). 2019 Dec;13(1):62-71. doi: 10.1080/19336950.2019.1568825.


    Flucher BE, Campiglio M. (2019) STAC proteins: The missing link in skeletal muscle EC coupling and new regulators of calcium channel function. Biochim Biophys Acta Mol Cell Res. 2019 Jul;1866(7):1101-1110. doi: 10.1016/j.bbamcr.2018.12.004. Epub 2018 Dec 10. Review.


    Campiglio M, Kaplan MM, Flucher BE. (2018) STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor. J Cell Physiol. 2018 Dec;233(12):9045-9051. doi: 10.1002/jcp.26767. Epub 2018 Aug 2.


    Costé de Bagneaux P, Campiglio M, Benedetti B, Tuluc P, Flucher BE. (2018) Role of putative voltage-sensor countercharge D4 in regulating gating properties of CaV1.2 and CaV1.3 calcium channels. Channels (Austin). 2018;12(1):249-261. doi: 10.1080/19336950.2018.1482183.


    Kaplan MM, Sultana N, Benedetti A, Obermair GJ, Linde NF, Papadopoulos S, Dayal A, Grabner M, Flucher BE. (2018) Calcium Influx and Release Cooperatively Regulate AChR Patterning and Motor Axon Outgrowth during Neuromuscular Junction Formation. Cell Rep. 2018 Jun 26;23(13):3891-3904. doi: 10.1016/j.celrep.2018.05.085.


    Campiglio M, Costé de Bagneaux P, Ortner NJ, Tuluc P, Van Petegem F, Flucher BE. (2018) STAC proteins associate to the IQ domain of CaV1.2 and inhibit calcium-dependent inactivation. Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):1376-1381. doi: 10.1073/pnas.1715997115. Epub 2018 Jan 23.


    Wong King Yuen SM, Campiglio M, Tung CC, Flucher BE, Van Petegem F. (2017) Structural insights into binding of STAC proteins to voltage-gated calcium channels. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):E9520-E9528. doi: 10.1073/pnas.1708852114. Epub 2017 Oct 23.


    Findeisen, F., Campiglio, M., Jo, H., Rumpf, C.H., Pope, L., Abderemane-Ali, F., Rossen, N.D., Flucher, B.E., DeGrado, W.F., and Minor, D.L. Jr (2017) Stapled voltage-gated calcium channel (CaV) α-interaction domain (AID) peptides act as selective protein-protein interaction inhibitors of CaV function. ACS Chem. Neurosci., 2017 Mar 17. doi: 10.1021/acschemneuro.6b00454. [Epub ahead of print]


    Mastrolia, V., Flucher, S.M., Obermair, G.J., Drach, M., Hofer, H., Renström, E., Schwartz, A., Striessnig, J., Flucher, B.E., and Tuluc, P. (2017) Loss of α2δ-1 calcium channel subunit function increases the susceptibility for diabetes. Diabetes, 2017 Jan 23. pii: db151349. doi: 10.2337/db16-0336. [Epub ahead of print]


    Campiglio, M. and Flucher, B.E. (2017) STAC3 stably interacts through its C1 domain with CaV1.1 in skeletal muscle triads. Sci. Rep., 7:41003. doi: 10.1038/srep41003;


    Flucher, B.E. and Tuluc, P. (2017) How and why are calcium currents curtailed in the skeletal muscle voltage-gated calcium channels? J. Physiol., 595:1451-1463.


    Stanika, R., Campiglio, M., Pinggera, A., Lee, A., Striessnig, J.,  Flucher, B.E., and Obermair, G.J. (2016) Splice variants of the Cav1.3 L-type calcium channel regulate dendritic spine morphology. Sci. Rep., 2016 Oct 6;6:34528. doi: 10.1038/srep34528;


    Flucher, B.E. (2016) Specific contributions of the four voltage-sensing domains in L-type calcium channels to gating and modulation J. Gen. Physiol. 148:91-95.


    Rzhepetskyy, Y., Lazniewska, J., Proft, J.,  Campiglio, M., Flucher, B.E., and Weiss, N. (2016) A CaV3.2/Stac1 molecular complex controls T-type channel expression at the plasma membrane. Channels, 10:346-354.


    Tuluc, P., Benedetti, B., Coste de Bagneaux, P., Grabner, M., and Flucher, B.E. (2016) Two distinct voltage sensing domains control voltage-sensitivity and kinetics of current activation in CaV1.1 calcium channels. J. Gen. Physiology, 147:437-449.


    Benedetti, B., Benedetti, A., and Flucher, B.E. (2016) Loss of the calcium channel β4 subunit impairs parallel fiber volley and purkinje cell firing in cerebellum of adult ataxic mice. Eur.  J. Neurosci. 43:1486-1498.


    Flucher, B.E. (2016) Retrograde coupling: Muscle’s orphan signaling pathway? Biophs. J. 110:870-871.


    Sultana, N., Dienes, B., Benedetti, A., Tuluc, P., Szentesi, P., Sztretye, M., Rainer, J., Hess, M.W., Schwarzer, C., Obermair, G.J., Csernoch, L., and Flucher, B.E. (2016) Restricting calcium currents is required for correct fiber type specification in skeletal muscle. Development, 143:1547-1559.


    Tuluc, P., Yarov-Yarovoi, V., Benedetti, B., and Flucher, B.E. (2015) Molecular interactions in the voltage sensor controlling gating properties of CaV calcium channels. Structure, 24:261-271.


    Kaur, G., Pinggera, A., Ortner, N.J., Lieb, A., Sinnegger-Brauns, M.J., Yarov-Yarovoy, V., Obermair, G.J., Flucher, B.E., Striessnig, J. (2015) A polybasic plasma membrane binding motif in the I-II linker stabilizes voltage-gated Cav1.2 calcium channel function. J. Biol. Chem. 290:21086-100.


    Flucher, B.E. (2015) How is SR calcium release in muscle modulated by PIP(4,5)2? J. Gen. Physiol. 145:361-364.


    Campiglio, M. and Flucher, B.E., The Role of Auxiliary Subunits for the Functional Diversity of Voltage-Gated Calcium Channels. J Cell Physiol, 2015.


    Benedetti, B., Tuluc, P., Mastrolia, V., Dlaska, C., and Flucher, B.E., Physiological and Pharmacological Modulation of the Embryonic Skeletal Muscle Calcium Channel Splice Variant CaV1.1e. Biophys J, 2015. 108(5): p. 1072-80.


    Etemad, S., Obermair, G.J., Bindreither, D., Benedetti, A., Stanika, R., Di Biase, V., Burtscher, V., Koschak, A., Kofler, R., Geley, S., Wille, A., Lusser, A., Flockerzi, V., and Flucher, B.E., Differential neuronal targeting of a new and two known calcium channel beta4 subunit splice variants correlates with their regulation of gene expression. J Neurosci, 2014. 34(4): p. 1446-61.


    Gstir, R., Schafferer, S., Scheideler, M., Misslinger, M., Griehl, M., Daschil, N., Humpel, C., Obermair, G.J., Schmuckermair, C., Striessnig, J., Flucher, B.E., and Huttenhofer, A., Generation of a neuro-specific microarray reveals novel differentially expressed noncoding RNAs in mouse models for neurodegenerative diseases. RNA, 2014. 20(12): p. 1929-43.


    Etemad, S., Campiglio, M., Obermair, G.J., and Flucher, B.E., The juvenile myoclonic epilepsy mutant of the calcium channel beta(4) subunit displays normal nuclear targeting in nerve and muscle cells. Channels (Austin), 2014. 8(4): p. 334-43.


    Campiglio, M., Di Biase, V., Tuluc, P., and Flucher, B.E., Stable incorporation versus dynamic exchange of beta subunits in a native Ca2+ channel complex. J Cell Sci, 2013. 126(Pt 9): p. 2092-101.


    Nimmervoll, B., Flucher, B.E., and Obermair, G.J., Dominance of P/Q-type calcium channels in depolarization-induced presynaptic FM dye release in cultured hippocampal neurons. Neuroscience, 2013. 253: p. 330-40.


    Daschil, N., Obermair, G.J., Flucher, B.E., Stefanova, N., Hutter-Paier, B., Windisch, M., Humpel, C., and Marksteiner, J., CaV1.2 calcium channel expression in reactive astrocytes is associated with the formation of amyloid-beta plaques in an Alzheimer's disease mouse model. J Alzheimers Dis, 2013. 37(2): p. 439-51.


    Nakada, T., Flucher, B.E., Kashihara, T., Sheng, X., Shibazaki, T., Horiuchi-Hirose, M., Gomi, S., Hirose, M., and Yamada, M., The proximal C-terminus of alpha(1C) subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels. Biochem J, 2012. 448(2): p. 221-31.


    Di Biase, V., Tuluc, P., Campiglio, M., Obermair, G.J., Heine, M., and Flucher, B.E., Surface traffic of dendritic CaV1.2 calcium channels in hippocampal neurons. J Neurosci, 2011. 31(38): p. 13682-94.


    Flucher, B.E. and Tuluc, P., A new L-type calcium channel isoform required for normal patterning of the developing neuromuscular junction. Channels (Austin), 2011. 5(6): p. 518-24.


    Tuluc, P. and Flucher, B.E., Divergent biophysical properties, gating mechanisms, and possible functions of the two skeletal muscle Ca(V)1.1 calcium channel splice variants. J Muscle Res Cell Motil, 2011. 32(4-5): p. 249-56.


    Schlick, B., Flucher, B.E., and Obermair, G.J., Voltage-activated calcium channel expression profiles in mouse brain and cultured hippocampal neurons. Neuroscience, 2010. 167(3): p. 786-98.


    Flucher, B.E., Rem-induced inhibition of Ca2+ channels--a three-pronged assault. J Physiol, 2010. 588(Pt 11): p. 1801-2.


    Obermair, G.J., Schlick, B., Di Biase, V., Subramanyam, P., Gebhart, M., Baumgartner, S., and Flucher, B.E., Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons. J Biol Chem, 2010. 285(8): p. 5776-91.


    Pirone, A., Schredelseker, J., Tuluc, P., Gravino, E., Fortunato, G., Flucher, B.E., Carsana, A., Salvatore, F., and Grabner, M., Identification and functional characterization of malignant hyperthermia mutation T1354S in the outer pore of the Cavalpha1S-subunit. Am J Physiol Cell Physiol, 2010. 299(6): p. C1345-54.


    Subramanyam, P., Obermair, G.J., Baumgartner, S., Gebhart, M., Striessnig, J., Kaufmann, W.A., Geley, S., and Flucher, B.E., Activity and calcium regulate nuclear targeting of the calcium channel beta4b subunit in nerve and muscle cells. Channels (Austin), 2009. 3(5): p. 343-55.


    Mackrill, J.J., England, K., Flucher, B.E., and McCarthy, T.V., 'Shotgun immunological' approach for analysis of a complex subcellular system. Int J Mol Med, 2009. 23(3): p. 399-405.


    Di Biase, V., Flucher, B.E., and Obermair, G.J., Resolving sub-synaptic compartments with double immunofluorescence labeling in hippocampal neurons. J Neurosci Methods, 2009. 176(2): p. 78-84.


    Tuluc, P., Molenda, N., Schlick, B., Obermair, G.J., Flucher, B.E., and Jurkat-Rott, K., A CaV1.1 Ca2+ channel splice variant with high conductance and voltage-sensitivity alters EC coupling in developing skeletal muscle. Biophys J, 2009. 96(1): p. 35-44.


    Obermair, G.J., Tuluc, P., and Flucher, B.E., Auxiliary Ca(2+) channel subunits: lessons learned from muscle. Curr Opin Pharmacol, 2008. 8(3): p. 311-8.


    Di Biase, V., Obermair, G.J., Szabo, Z., Altier, C., Sanguesa, J., Bourinet, E., and Flucher, B.E., Stable membrane expression of postsynaptic CaV1.2 calcium channel clusters is independent of interactions with AKAP79/150 and PDZ proteins. J Neurosci, 2008. 28(51): p. 13845-55.


    Bleunven, C., Treves, S., Jinyu, X., Leo, E., Ronjat, M., De Waard, M., Kern, G., Flucher, B.E., and Zorzato, F., SRP-27 is a novel component of the supramolecular signalling complex involved in skeletal muscle excitation-contraction coupling. Biochem J, 2008. 411(2): p. 343-9.


    Tuluc, P., Kern, G., Obermair, G.J., and Flucher, B.E., Computer modeling of siRNA knockdown effects indicates an essential role of the Ca2+ channel alpha2delta-1 subunit in cardiac excitation-contraction coupling. Proc Natl Acad Sci U S A, 2007. 104(26): p. 11091-6.


    Szabo, Z., Obermair, G.J., Cooper, C.B., Zamponi, G.W., and Flucher, B.E., Role of the synprint site in presynaptic targeting of the calcium channel CaV2.2 in hippocampal neurons. Eur J Neurosci, 2006. 24(3): p. 709-18.


    Schredelseker, J., Di Biase, V., Obermair, G.J., Felder, E.T., Flucher, B.E., Franzini-Armstrong, C., and Grabner, M., The beta 1a subunit is essential for the assembly of dihydropyridine-receptor arrays in skeletal muscle. Proc Natl Acad Sci U S A, 2005. 102(47): p. 17219-24.


    Schuhmeier, R.P., Gouadon, E., Ursu, D., Kasielke, N., Flucher, B.E., Grabner, M., and Melzer, W., Functional interaction of CaV channel isoforms with ryanodine receptors studied in dysgenic myotubes. Biophys J, 2005. 88(3): p. 1765-77.


    Obermair, G.J., Kugler, G., Baumgartner, S., Tuluc, P., Grabner, M., and Flucher, B.E., The Ca2+ channel alpha2delta-1 subunit determines Ca2+ current kinetics in skeletal muscle but not targeting of alpha1S or excitation-contraction coupling. J Biol Chem, 2005. 280(3): p. 2229-37.


    Kern, G. and Flucher, B.E., Localization of transgenes and genotyping of H-2kb-tsA58 transgenic mice. Biotechniques, 2005. 38(1): p. 38, 40, 42.


    Flucher, B.E., Obermair, G.J., Tuluc, P., Schredelseker, J., Kern, G., and Grabner, M., The role of auxiliary dihydropyridine receptor subunits in muscle. J Muscle Res Cell Motil, 2005. 26(1): p. 1-6.


    Weiss, R.G., O'Connell, K.M., Flucher, B.E., Allen, P.D., Grabner, M., and Dirksen, R.T., Functional analysis of the R1086H malignant hyperthermia mutation in the DHPR reveals an unexpected influence of the III-IV loop on skeletal muscle EC coupling. Am J Physiol Cell Physiol, 2004. 287(4): p. C1094-102.


    Kugler, G., Grabner, M., Platzer, J., Striessnig, J., and Flucher, B.E., The monoclonal antibody mAB 1A binds to the excitation--contraction coupling domain in the II-III loop of the skeletal muscle calcium channel alpha(1S) subunit. Arch Biochem Biophys, 2004. 427(1): p. 91-100.


    Kugler, G., Weiss, R.G., Flucher, B.E., and Grabner, M., Structural requirements of the dihydropyridine receptor alpha1S II-III loop for skeletal-type excitation-contraction coupling. J Biol Chem, 2004. 279(6): p. 4721-8.


    Takekura, H., Paolini, C., Franzini-Armstrong, C., Kugler, G., Grabner, M., and Flucher, B.E., Differential contribution of skeletal and cardiac II-III loop sequences to the assembly of dihydropyridine-receptor arrays in skeletal muscle. Mol Biol Cell, 2004. 15(12): p. 5408-19.


    Obermair, G.J., Szabo, Z., Bourinet, E., and Flucher, B.E., Differential targeting of the L-type Ca2+ channel alpha 1C (CaV1.2) to synaptic and extrasynaptic compartments in hippocampal neurons. Eur J Neurosci, 2004. 19(8): p. 2109-22.


    Obermair, G.J., Kugler, G., and Flucher, B.E., The role of the calcium channel alpha 2 delta-1 subunit in skeletal muscle. J Muscle Res Cell Motil, 2004. 25(3): p. 239-40.


    Kasielke, N., Obermair, G.J., Kugler, G., Grabner, M., and Flucher, B.E., Cardiac-type EC-coupling in dysgenic myotubes restored with Ca2+ channel subunit isoforms alpha1C and alpha1D does not correlate with current density. Biophys J, 2003. 84(6): p. 3816-28.


    Obermair, G.J., Kaufmann, W.A., Knaus, H.G., and Flucher, B.E., The small conductance Ca2+-activated K+ channel SK3 is localized in nerve terminals of excitatory synapses of cultured mouse hippocampal neurons. Eur J Neurosci, 2003. 17(4): p. 721-31.


    Hitzl, M., Striessnig, J., Neuhuber, B., and Flucher, B.E., A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site. FEBS Lett, 2002. 524(1-3): p. 188-92.


    Flucher, B.E., Weiss, R.G., and Grabner, M., Cooperation of two-domain Ca(2+) channel fragments in triad targeting and restoration of excitation- contraction coupling in skeletal muscle. Proc Natl Acad Sci U S A, 2002. 99(15): p. 10167-72.


    Takekura, H., Flucher, B.E., and Franzini-Armstrong, C., Sequential docking, molecular differentiation, and positioning of T-Tubule/SR junctions in developing mouse skeletal muscle. Dev Biol, 2001. 239(2): p. 204-14.


    Wilkens, C.M., Kasielke, N., Flucher, B.E., Beam, K.G., and Grabner, M., Excitation-contraction coupling is unaffected by drastic alteration of the sequence surrounding residues L720-L764 of the alpha 1S II-III loop. Proc Natl Acad Sci U S A, 2001. 98(10): p. 5892-7.


    Flucher, B.E., Kasielke, N., and Grabner, M., The triad targeting signal of the skeletal muscle calcium channel is localized in the COOH terminus of the alpha(1S) subunit. J Cell Biol, 2000. 151(2): p. 467-78.


    Flucher, B.E., Kasielke, N., Gerster, U., Neuhuber, B., and Grabner, M., Insertion of the full-length calcium channel alpha(1S) subunit into triads of skeletal muscle in vitro. FEBS Lett, 2000. 474(1): p. 93-8.


    Sipos, I., Pika-Hartlaub, U., Hofmann, F., Flucher, B.E., and Melzer, W., Effects of the dihydropyridine receptor subunits gamma and alpha2delta on the kinetics of heterologously expressed L-type Ca2+ channels. Pflugers Arch, 2000. 439(6): p. 691-9.


    Gerster, U., Neuhuber, B., Groschner, K., Striessnig, J., and Flucher, B.E., Current modulation and membrane targeting of the calcium channel alpha1C subunit are independent functions of the beta subunit. J Physiol, 1999. 517 ( Pt 2): p. 353-68.


    Flucher, B.E., Conti, A., Takeshima, H., and Sorrentino, V., Type 3 and type 1 ryanodine receptors are localized in triads of the same mammalian skeletal muscle fibers. J Cell Biol, 1999. 146(3): p. 621-30.


    Neuhuber, B., Gerster, U., Doring, F., Glossmann, H., Tanabe, T., and Flucher, B.E., Association of calcium channel alpha1S and beta1a subunits is required for the targeting of beta1a but not of alpha1S into skeletal muscle triads. Proc Natl Acad Sci U S A, 1998. 95(9): p. 5015-20.


    Neuhuber, B., Gerster, U., Mitterdorfer, J., Glossmann, H., and Flucher, B.E., Differential effects of Ca2+ channel beta1a and beta2a subunits on complex formation with alpha1S and on current expression in tsA201 cells. J Biol Chem, 1998. 273(15): p. 9110-8.


    Protasi, F., Franzini-Armstrong, C., and Flucher, B.E., Coordinated incorporation of skeletal muscle dihydropyridine receptors and ryanodine receptors in peripheral couplings of BC3H1 cells. J Cell Biol, 1997. 137(4): p. 859-70.


    Flucher, B.E. and Franzini-Armstrong, C., Formation of junctions involved in excitation-contraction coupling in skeletal and cardiac muscle. Proc Natl Acad Sci U S A, 1996. 93(15): p. 8101-6.


    Powell, J.A., Petherbridge, L., and Flucher, B.E., Formation of triads without the dihydropyridine receptor alpha subunits in cell lines from dysgenic skeletal muscle. J Cell Biol, 1996. 134(2): p. 375-87.


    Flucher, B.E., Andrews, S.B., and Daniels, M.P., Molecular organization of transverse tubule/sarcoplasmic reticulum junctions during development of excitation-contraction coupling in skeletal muscle. Mol Biol Cell, 1994. 5(10): p. 1105-18.


    Huang, C.F., Flucher, B.E., Schmidt, M.M., Stroud, S.K., and Schmidt, J., Depolarization-transcription signals in skeletal muscle use calcium flux through L channels, but bypass the sarcoplasmic reticulum. Neuron, 1994. 13(1): p. 167-77.


    Flucher, B.E., Takekura, H., and Franzini-Armstrong, C., Development of the excitation-contraction coupling apparatus in skeletal muscle: association of sarcoplasmic reticulum and transverse tubules with myofibrils. Dev Biol, 1993. 160(1): p. 135-47.


    Fowler, V.M., Sussmann, M.A., Miller, P.G., Flucher, B.E., and Daniels, M.P., Tropomodulin is associated with the free (pointed) ends of the thin filaments in rat skeletal muscle. J Cell Biol, 1993. 120(2): p. 411-20.


    Flucher, B.E., Andrews, S.B., Fleischer, S., Marks, A.R., Caswell, A., and Powell, J.A., Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro. J Cell Biol, 1993. 123(5): p. 1161-74.


    Flucher, B.E. and Andrews, S.B., Characterization of spontaneous and action potential-induced calcium transients in developing myotubes in vitro. Cell Motil Cytoskeleton, 1993. 25(2): p. 143-57.


    Flucher, B.E., Structural analysis of muscle development: transverse tubules, sarcoplasmic reticulum, and the triad. Dev Biol, 1992. 154(2): p. 245-60.


    Flucher, B.E., Phillips, J.L., Powell, J.A., Andrews, S.B., and Daniels, M.P., Coordinated development of myofibrils, sarcoplasmic reticulum and transverse tubules in normal and dysgenic mouse skeletal muscle, in vivo and in vitro. Dev Biol, 1992. 150(2): p. 266-80.


    Flucher, B.E., Terasaki, M., Chin, H.M., Beeler, T.J., and Daniels, M.P., Biogenesis of transverse tubules in skeletal muscle in vitro. Dev Biol, 1991. 145(1): p. 77-90.


    Flucher, B.E., Phillips, J.L., and Powell, J.A., Dihydropyridine receptor alpha subunits in normal and dysgenic muscle in vitro: expression of alpha 1 is required for proper targeting and distribution of alpha 2. J Cell Biol, 1991. 115(5): p. 1345-56.


    Flucher, B.E., Morton, M.E., Froehner, S.C., and Daniels, M.P., Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads. Neuron, 1990. 5(3): p. 339-51.


    Flucher, B.E. and Daniels, M.P., Distribution of Na+ channels and ankyrin in neuromuscular junctions is complementary to that of acetylcholine receptors and the 43 kd protein. Neuron, 1989. 3(2): p. 163-75.


    Flucher, B.E., Lenglachner-Bachinger, C., and Feurle, G.E., Immunocytochemical evidence for the colocalization of neurotensin/xenopsin- and gastrin/caerulein-immunoreactive substances in Xenopus laevis gastrointestinal tract. Gen Comp Endocrinol, 1988. 72(1): p. 54-62.


    Flucher, B.E., Lenglachner-Bachinger, C., Pohlhammer, K., Adam, H., and Mollay, C., Skin peptides in Xenopus laevis: morphological requirements for precursor processing in developing and regenerating granular skin glands. J Cell Biol, 1986. 103(6 Pt 1): p. 2299-309.