Capillary Electrophoresis
Equipment:
PA 800 Plus Pharmaceutical Analysis System (Sciex) equipped with UV absorbance and diode array detectors.
Method:
Capillary electrophoresis (CE) is a family of electrokinetic separation methods, all of them applicable for the analysis of proteins and peptides. CE combines the advantages of conventional gel electrophoresis and high-performance liquid chromatography to separate solutes according to their ability to migrate in an electric field inside a fused silica capillary. It employs narrow-bore (20-200 µm i.d.) capillaries to perform separations of both large and small molecules. The instrumental configuration for CE is relatively simple. All that is required is a fused-silica capillary with an optical viewing window, a controllable high voltage power supply, two electrode assemblies, two buffer reservoirs, and a detector. Method-inherent advantages are highly efficient separations, on-line detection, precise quantification, and automation. Separation modes available in our lab are capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE).
Sample Preparation:
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Samples can either be dissolved in water (most suitable) or in buffers with known composition, pH value etc.
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Samples can also be lyophilized.
Provided Services:
- Quality control of purified proteins (e. g. Monoclonal Antibodies) or peptides with focus on
- Purity/Heterogeneity
- posttranslational modifications (e.g. glycosylation, phosphorylation, ...)
- Quality control of Plasmids and Nucleic Acid Therapeutics
- Analysis of viruses, subviral particles and virus complexes