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Renal Epithelial Cell and Tissue Culture

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  1. Rauch C., Feifel E., Flörl A., Pfaller K. and Gstraunthaler G.. Human Platelet Lysates Promote the Differentiation Potential of Adipose-Derived Adult Stem Cell Cultures. Journal of Advanced Biotechnology and Bioenineering. 2014  2 (2). P 39-48. dx.doi.org/10.12970/2311-1755.2014.02.02. http://www.synergypublishers.com/downloads/jabbv2n2a1/
  2. Gstraunthaler, G., Lindl, T., and van der Valk, J., A severe case of fraudulent blending of fetal bovine serum strengthens the case for serum-free cell and tissue culture applications. Altern Lab Anim, 2014. 42(3): p. 207-9. http://www.ncbi.nlm.nih.gov/pubmed/25068932
  3. Curthoys, N.P. and Gstraunthaler, G., pH-responsive, gluconeogenic renal epithelial LLC-PK1-FBPase+cells: a versatile in vitro model to study renal proximal tubule metabolism and function. Am J Physiol Renal Physiol, 2014. 307(1): p. F1-f11. http://www.ncbi.nlm.nih.gov/pubmed/24808535
  4. Rauch, C., Wechselberger, J., Feifel, E., and Gstraunthaler, G., Human Platelet Lysates Successfully Replace Fetal Bovine Serum in Adipose-Derived Adult Stem Cell Culture. Journal of Advanced Biotechnology and Bioengineering, 2014. 2(1): p. 1-11. http://www.synergypublishers.com/?did=9610&vp_edd_act=show_download
  5. Aschauer, L., Gruber, L.N., Pfaller, W., Limonciel, A., Athersuch, T.J., Cavill, R., Khan, A., Gstraunthaler, G., Grillari, J., Grillari, R., Hewitt, P., Leonard, M.O., Wilmes, A., and Jennings, P., Delineation of the key aspects in the regulation of epithelial monolayer formation. Mol Cell Biol, 2013. 33(13): p. 2535-50. http://www.ncbi.nlm.nih.gov/pubmed/23608536
  6. Gstraunthaler, G., Lindl, T., and van der Valk, J., A plea to reduce or replace fetal bovine serum in cell culture media. Cytotechnology, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23975256
  7. Kronthaler, J., Gstraunthaler, G., and Heel, C., Optimizing high-throughput metabolomic biomarker screening: a study of quenching solutions to freeze intracellular metabolism in CHO cells. OMICS, 2012. 16(3): p. 90-7. http://www.ncbi.nlm.nih.gov/pubmed/22401654
  8. Rauch, C., Feifel, E., Amann, E.M., Spotl, H.P., Schennach, H., Pfaller, W., and Gstraunthaler, G., Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX, 2011. 28(4): p. 305-16. http://www.ncbi.nlm.nih.gov/pubmed/22130485
  9. van der Valk, J., Brunner, D., De Smet, K., Fex Svenningsen, A., Honegger, P., Knudsen, L.E., Lindl, T., Noraberg, J., Price, A., Scarino, M.L., and Gstraunthaler, G., Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro, 2010. 24(4): p. 1053-63. http://www.ncbi.nlm.nih.gov/pubmed/20362047
  10. Brunner, D., Frank, J., Appl, H., Schoffl, H., Pfaller, W., and Gstraunthaler, G., Serum-free cell culture: the serum-free media interactive online database. ALTEX, 2010. 27(1): p. 53-62. http://www.ncbi.nlm.nih.gov/pubmed/20390239
  11. Andratsch, M., Feifel, E., Taylor, L., O'Hayre, M., Schramek, H., Curthoys, N.P., and Gstraunthaler, G., TGF-beta signaling and its effect on glutaminase expression in LLC-PK1-FBPase+ cells. Am J Physiol Renal Physiol, 2007. 293(3): p. F846-53. http://www.ncbi.nlm.nih.gov/pubmed/17596530
  12. Pollack, V., Sarkozi, R., Banki, Z., Feifel, E., Wehn, S., Gstraunthaler, G., Stoiber, H., Mayer, G., Montesano, R., Strutz, F., and Schramek, H., Oncostatin M-induced effects on EMT in human proximal tubular cells: differential role of ERK signaling. Am J Physiol Renal Physiol, 2007. 293(5): p. F1714-26. http://www.ncbi.nlm.nih.gov/pubmed/17881458
  13. O'Hayre, M., Taylor, L., Andratsch, M., Feifel, E., Gstraunthaler, G., and Curthoys, N.P., Effects of constitutively active and dominant negative MAPK kinase (MKK) 3 and MKK6 on the pH-responsive increase in phosphoenolpyruvate carboxykinase mRNA. J Biol Chem, 2006. 281(5): p. 2982-8. http://www.ncbi.nlm.nih.gov/pubmed/16319064
  14. Coecke, S., Balls, M., Bowe, G., Davis, J., Gstraunthaler, G., Hartung, T., Hay, R., Merten, O.W., Price, A., Schechtman, L., Stacey, G., Stokes, W., and Second, E.T.F.o.G.C.C.P., Guidance on good cell culture practice. a report of the second ECVAM task force on good cell culture practice. Altern Lab Anim, 2005. 33(3): p. 261-87. http://www.ncbi.nlm.nih.gov/pubmed/16180980
  15. van der Valk, J., Mellor, D., Brands, R., Fischer, R., Gruber, F., Gstraunthaler, G., Hellebrekers, L., Hyllner, J., Jonker, F.H., Prieto, P., Thalen, M., and Baumans, V., The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol In Vitro, 2004. 18(1): p. 1-12. http://www.ncbi.nlm.nih.gov/pubmed/14630056
  16. Schramek, H., Feifel, E., Marschitz, I., Golochtchapova, N., Gstraunthaler, G., and Montesano, R., Loss of active MEK1-ERK1/2 restores epithelial phenotype and morphogenesis in transdifferentiated MDCK cells. Am J Physiol Cell Physiol, 2003. 285(3): p. C652-61. http://www.ncbi.nlm.nih.gov/pubmed/12900389
  17. Gstraunthaler, G., Alternatives to the use of fetal bovine serum: serum-free cell culture. ALTEX, 2003. 20(4): p. 275-81. http://www.ncbi.nlm.nih.gov/pubmed/14671707
  18. Feifel, E., Obexer, P., Andratsch, M., Euler, S., Taylor, L., Tang, A., Wei, Y., Schramek, H., Curthoys, N.P., and Gstraunthaler, G., p38 MAPK mediates acid-induced transcription of PEPCK in LLC-PK(1)-FBPase(+) cells. Am J Physiol Renal Physiol, 2002. 283(4): p. F678-88. http://www.ncbi.nlm.nih.gov/pubmed/12217859
  19. Hartung, T., Balls, M., Bardouille, C., Blanck, O., Coecke, S., Gstraunthaler, G., Lewis, D., and Force, E.G.C.C.P.T., Good Cell Culture Practice. ECVAM Good Cell Culture Practice Task Force Report 1. Altern Lab Anim, 2002. 30(4): p. 407-14. http://www.ncbi.nlm.nih.gov/pubmed/12234246
  20. Curthoys, N.P. and Gstraunthaler, G., Mechanism of increased renal gene expression during metabolic acidosis. Am J Physiol Renal Physiol, 2001. 281(3): p. F381-90. http://www.ncbi.nlm.nih.gov/pubmed/11502586
  21. Hartung, T., Gstraunthaler, G., Coecke, S., Lewis, D., Blanck, O., and Balls, M., [Good cell culture practice (GCCP)--an initiative for standardization and quality control of in vitro studies. The establishment of an ECVAM Task Force on GCCP]. ALTEX, 2001. 18(1): p. 75-8. http://www.ncbi.nlm.nih.gov/pubmed/11248855
  22. Curthoys, N.P., Tang, A., and Gstraunthaler, G., pH regulation of renal gene expression. Novartis Found Symp, 2001. 240: p. 100-11; discussion 111-4. http://www.ncbi.nlm.nih.gov/pubmed/11727924
  23. Gstraunthaler, G., Holcomb, T., Feifel, E., Liu, W., Spitaler, N., and Curthoys, N.P., Differential expression and acid-base regulation of glutaminase mRNAs in gluconeogenic LLC-PK(1)-FBPase(+) cells. Am J Physiol Renal Physiol, 2000. 278(2): p. F227-37. http://www.ncbi.nlm.nih.gov/pubmed/10662727
  24. Gstraunthaler, G., Seppi, T., and Pfaller, W., Impact of culture conditions, culture media volumes, and glucose content on metabolic properties of renal epithelial cell cultures. Are renal cells in tissue culture hypoxic? Cell Physiol Biochem, 1999. 9(3): p. 150-72. http://www.ncbi.nlm.nih.gov/pubmed/10494029
  25. Liu, W., Feifel, E., Holcomb, T., Liu, X., Spitaler, N., Gstraunthaler, G., and Curthoys, N.P., PMA and staurosporine affect expression of the PCK gene in LLC-PK1-F+ cells. Am J Physiol, 1998. 275(3 Pt 2): p. F361-9. http://www.ncbi.nlm.nih.gov/pubmed/9729508
  26. Pfaller, W. and Gstraunthaler, G., Nephrotoxicity testing in vitro--what we know and what we need to know. Environ Health Perspect, 1998. 106 Suppl 2: p. 559-69. http://www.ncbi.nlm.nih.gov/pubmed/9599703
  27. Willinger, C.C., Thamaree, S., Schramek, H., Gstraunthaler, G., and Pfaller, W., In vitro nephrotoxicity of Russell's viper venom. Kidney Int, 1995. 47(2): p. 518-28. http://www.ncbi.nlm.nih.gov/pubmed/7723237
  28. Steinmassl, D., Pfaller, W., Gstraunthaler, G., and Hoffmann, W., LLC-PK1 epithelia as a model for in vitro assessment of proximal tubular nephrotoxicity. In Vitro Cell Dev Biol Anim, 1995. 31(2): p. 94-106. http://www.ncbi.nlm.nih.gov/pubmed/7735573
  29. Holcomb, T., Curthoys, N.P., and Gstraunthaler, G., Subcellular localization of PEPCK and metabolism of gluconeogenic substrains of renal cell lines. Am J Physiol, 1995. 268(2 Pt 1): p. C449-57. http://www.ncbi.nlm.nih.gov/pubmed/7864084
  30. Gstraunthaler, G.J., Ammoniagenesis in renal cell culture. A comparative study on ammonia metabolism of renal epithelial cell lines. Contrib Nephrol, 1994. 110: p. 88-97. http://www.ncbi.nlm.nih.gov/pubmed/7956263
  31. Netzer, A. and Gstraunthaler, G., Selective release of apical membrane enzymes from cultured renal epithelia by phosphatidylinositol-specific phospholipase C. Ren Physiol Biochem, 1993. 16(6): p. 299-310. http://www.ncbi.nlm.nih.gov/pubmed/7506439
  32. Eberharter, A., Grabher, A., Gstraunthaler, G., and Loidl, P., Nuclear matrix of the lower eukaryote Physarum polycephalum and the mammalian epithelial LLC-PK1 cell line. A comprehensive investigation of different preparation procedures. Eur J Biochem, 1993. 212(2): p. 573-80. http://www.ncbi.nlm.nih.gov/pubmed/7680312
  33. Gstraunthaler, G., Landauer, F., and Pfaller, W., Ammoniagenesis in renal cell culture. Lack of extracellular ammoniagenesis at the apical surface of LLC-PK1 epithelia. Ren Physiol Biochem, 1993. 16(4): p. 203-11. http://www.ncbi.nlm.nih.gov/pubmed/7689242
  34. Schramek, H., Gstraunthaler, G., Willinger, C.C., and Pfaller, W., Hyperosmolality regulates endothelin release by Madin-Darby canine kidney cells. J Am Soc Nephrol, 1993. 4(2): p. 206-13. http://www.ncbi.nlm.nih.gov/pubmed/8400084
  35. Schramek, H., Willinger, C.C., Gstraunthaler, G., and Pfaller, W., Endothelin-3 modulates glomerular filtration rate in the isolated perfused rat kidney. Ren Physiol Biochem, 1992. 15(6): p. 325-33. http://www.ncbi.nlm.nih.gov/pubmed/1282726
  36. Gstraunthaler, G., Landauer, F., and Pfaller, W., Ammoniagenesis in LLC-PK1 cultures: role of transamination. Am J Physiol, 1992. 263(1 Pt 1): p. C47-54. http://www.ncbi.nlm.nih.gov/pubmed/1636683
  37. Pfaller, W., Gstraunthaler, G., and Willinger, C.C., Morphology of renal tubular damage from nephrotoxins. Toxicol Lett, 1990. 53(1-2): p. 39-43. http://www.ncbi.nlm.nih.gov/pubmed/2219185
  38. Gstraunthaler, G., Steinmassl, D., and Pfaller, W., Renal cell cultures: a tool for studying tubular function and nephrotoxicity. Toxicol Lett, 1990. 53(1-2): p. 1-7. http://www.ncbi.nlm.nih.gov/pubmed/2219147
  39. Gstraunthaler, G., Gersdorf, E., Fischer, W.M., Joannidis, M., and Pfaller, W., Morphological and biochemical changes of LLC-PK1 cells during adaptation to glucose-free culture conditions. Ren Physiol Biochem, 1990. 13(3): p. 137-53. http://www.ncbi.nlm.nih.gov/pubmed/1690907
  40. Oberleithner, H., Steigner, W., Silbernagl, S., Vogel, U., Gstraunthaler, G., and Pfaller, W., Madin-Darby canine kidney cells. III. Aldosterone stimulates an apical H+/K+ pump. Pflugers Arch, 1990. 416(5): p. 540-7. http://www.ncbi.nlm.nih.gov/pubmed/2172917
  41. Joannidis, M., Gstraunthaler, G., and Pfaller, W., Xanthine oxidase: evidence against a causative role in renal reperfusion injury. Am J Physiol, 1990. 258(2 Pt 2): p. F232-6. http://www.ncbi.nlm.nih.gov/pubmed/2309886
  42. Pfaller, W., Gstraunthaler, G., and Loidl, P., Morphology of the differentiation and maturation of LLC-PK1 epithelia. J Cell Physiol, 1990. 142(2): p. 247-54. http://www.ncbi.nlm.nih.gov/pubmed/2303524
  43. Pfaller, W., Gstraunthaler, G., Kersting, U., and Oberleithner, H., Carbonic anhydrase activity in Madin Darby canine kidney cells. Evidence for intercalated cell properties. Ren Physiol Biochem, 1989. 12(5-6): p. 328-37. http://www.ncbi.nlm.nih.gov/pubmed/2516353
  44. Frick, H., Gstraunthaler, G., and Pfaller, W., Modification of membrane protein expression and protein secretion in LLC-PK1 cultures grown on different carbohydrates. Ren Physiol Biochem, 1989. 12(5-6): p. 393-9. http://www.ncbi.nlm.nih.gov/pubmed/2576149
  45. Kersting, U., Joha, H., Steigner, W., Gassner, B., Gstraunthaler, G., Pfaller, W., and Oberleithner, H., Fusion of cultured dog kidney (MDCK) cells: I. Technique, fate of plasma membranes and of cell nuclei. J Membr Biol, 1989. 111(1): p. 37-48. http://www.ncbi.nlm.nih.gov/pubmed/2810350
  46. Ruedl, C., Gstraunthaler, G., and Moser, M., Differential inhibitory action of the fungal toxin orellanine on alkaline phosphatase isoenzymes. Biochim Biophys Acta, 1989. 991(2): p. 280-3. http://www.ncbi.nlm.nih.gov/pubmed/2566329
  47. Pfaller, W., Joannidis, M., Gstraunthaler, G., and Kotanko, P., Quantitative morphologic changes in nephron structures and urinary enzyme activity pattern in sodium-maleate-induced renal injury. Ren Physiol Biochem, 1989. 12(1): p. 56-64. http://www.ncbi.nlm.nih.gov/pubmed/2727385
  48. Gstraunthaler, G.J., Epithelial cells in tissue culture. Ren Physiol Biochem, 1988. 11(1-2): p. 1-42. http://www.ncbi.nlm.nih.gov/pubmed/3074393
  49. Gstraunthaler, G., Harris, H.W., Jr., and Handler, J.S., Precursors of ribose 5-phosphate suppress expression of glucose-regulated proteins in LLC-PK1 cells. Am J Physiol, 1987. 252(2 Pt 1): p. C239-43. http://www.ncbi.nlm.nih.gov/pubmed/3103465
  50. Gstraunthaler, G. and Handler, J.S., Isolation, growth, and characterization of a gluconeogenic strain of renal cells. Am J Physiol, 1987. 252(2 Pt 1): p. C232-8. http://www.ncbi.nlm.nih.gov/pubmed/3030122
  51. Kotanko, P., Keiler, R., Knabl, L., Aulitzky, W., Margreiter, R., Gstraunthaler, G., and Pfaller, W., Urinary enzyme analysis in renal allograft transplantation. Clin Chim Acta, 1986. 160(2): p. 137-44. http://www.ncbi.nlm.nih.gov/pubmed/3022970
  52. Paulmichl, M., Gstraunthaler, G., and Lang, F., Electrical properties of Madin-Darby canine kidney cells. Effects of extracellular potassium and bicarbonate. Pflugers Arch, 1985. 405(2): p. 102-7. http://www.ncbi.nlm.nih.gov/pubmed/2997696
  53. Gstraunthaler, G., Pfaller, W., and Kotanko, P., Lack of fructose-1,6-bisphosphatase activity in LLC-PK1 cells. Am J Physiol, 1985. 248(1 Pt 1): p. C181-3. http://www.ncbi.nlm.nih.gov/pubmed/2981475
  54. Gstraunthaler, G., Pfaller, W., and Kotanko, P., Biochemical characterization of renal epithelial cell cultures (LLC-PK1 and MDCK). Am J Physiol, 1985. 248(4 Pt 2): p. F536-44. http://www.ncbi.nlm.nih.gov/pubmed/3985161
  55. Gstraunthaler, G., Pfaller, W., and Kotanko, P., Interrelation between oxygen consumption and Na-K-ATPase activity in rat renal proximal tubule suspension. Ren Physiol, 1985. 8(1): p. 38-44. http://www.ncbi.nlm.nih.gov/pubmed/2982203
  56. Pfaller, W., Guder, W.G., Gstraunthaler, G., Kotanko, P., Jehart, I., and Purschel, S., Compartmentation of ATP within renal proximal tubular cells. Biochim Biophys Acta, 1984. 805(2): p. 152-7. http://www.ncbi.nlm.nih.gov/pubmed/6487658
  57. Kotanko, P., Gstraunthaler, G., and Pfaller, W., [Urinary enzymes in the non-invasive diagnosis of kidney epithelial lesions in acute kidney failure]. Wien Klin Wochenschr, 1984. 96(16): p. 625-9. http://www.ncbi.nlm.nih.gov/pubmed/6097054
  58. Pfaller, W., Gstraunthaler, G., Kotanko, P., Wolf, H., and Curthoys, N.P., Immunocytochemical localization of gamma-glutamyl-transferase on isolated renal cortical tubular fragments. Histochemistry, 1984. 80(3): p. 289-93. http://www.ncbi.nlm.nih.gov/pubmed/6144646
  59. Gstraunthaler, G., Pfaller, W., and Kotanko, P., Glutathione depletion and in vitro lipid peroxidation in mercury or maleate induced acute renal failure. Biochem Pharmacol, 1983. 32(19): p. 2969-72. http://www.ncbi.nlm.nih.gov/pubmed/6226293
  60. Semenitz, E., Casey, P.A., Pfaller, W., and Gstraunthaler, G., Microcalorimetric, turbidimetric, phase-contrast microscopic, and electron microscopic investigations of the actions of amoxicillin, clavulanic acid and augmentin on amoxicillin-sensitive and amoxicillin-resistant strains of Escherichia coli. Chemotherapy, 1983. 29(3): p. 192-207. http://www.ncbi.nlm.nih.gov/pubmed/6347546
  61. Lebek, G., Semenitz, E., Gstraunthaler, G., and Pfaller, W., [Comparative analysis of tetracycline, doxycycline and minocycline on chromosomal and plasmid tetracycline-resistant and -sensitive strains of Staphylococcus aureus using MIC, microcalorimetric effect and ultrastructural alterations]. Immun Infekt, 1982. 10(5): p. 193-201. http://www.ncbi.nlm.nih.gov/pubmed/7173974

Gerhard Gstraunthaler