Sektion für Neurobiochemie

 In order to make working in the biooptics facility as easy, convenient and fair as possible for everybody, it is necessary for all users to follow some basic rules.

General

The biooptics facility manager is: Priv. Doz. Dr. Martin Offterdinger

  • Before you use any system in the facility for the first time you have to have a personal introduction by the facility manager. This introduction is free of charge, extensive presence of the manager during a session (beyond answering questions) will be charged as "assisted usage".
  • Users are not allowed to introduce third persons to any system-this is exclusively done by the manager.
  • Please request an introduction only if you intend to use the instrument within the next few weeks.
  • After the introduction, users will sign a written document and get access to the booking system.
  • If you cannot remember how to operate a system you were already introduced to, ask for advice.
  • More extensive assisted usage (beyond a simple advice) can be booked online and will be charged.
  • Only samples classified in safety group S1 are allowed! Do not enter any facility room with samples classified above S1!
  • If you publish work done on one or more facility microscopes, please do include an acknowledgement like. 'We thank the biooptics facility of the MUI for their technical support.' 
  • Start and finish your experiments exactly on time.

Booking

  • Personal booking of the microscope before use is obligatory (Booking system).
  • No booking for other persons is allowed (guest/collaborators should contact me in advance).
  • Do not reserve the SP5 for more than 4 hours a day and twice per week, additional bookings are allowed on weekends and overnight(between18:00 and 9:00 h).
    • Exception: Last minute booking: If noone has booked the microscope after you 30 min before the end of your booked slot, you are allowed to extend your slot, BUT YOU HAVE TO BOOK IT, NEVERTHELESS, no working without booking, NEVER!
    • DMi8 may booked for longer time periods (days) since it is intened for long time lapses of living cells.
  • Long-term time-lapse experiments should be scheduled between 18:00 and 9:00 the next morning (please don´t forget to switch off devices not needed like the HBO lamp during overnight time-lapse on confocal systems).
  • You are responsible for the system while you are booked.
  • Delete your booking if you do not use your slot. If you delete the last booking on the booked day, ensure that the system is switched off (the previous user didn´t know that you are going to delete your booking).
  • Always include  the required lasers ("accessories", select).
  • Online booking data also serve as the basis for the cost contribution system.

Data

  • There is no backup of data saved on the local harddisk drives.
  • Store your freshly acquired data in D: in a folder labeled ‘user_phonenumer’.
  • You are not allowed to leave your image data permanently on the local harddisks!
  • Instrument setting data (<50 Mb) may be kept in a subfolder entitled 'settings'.
  • Transfer image data immediately at the end of your session using the group network drives (e.g. 'Neurobiochemie auf "share2adgroup"(P:)')
  • Users from clinical departments (TILAK net) can also use the network drives; they need to manually mount them on their local computers according to the following procedures.
  • In case of local memory shortage the manager will delete image data files older than 2 days.

Operation

Before you start:

  • Check the equipment for any obvious damage or spoilage and note it in the logbook.
  • Room temperature:  22°C = optimal temperature, do not change!
  • Switch off all mobile phones during work in the room M01.381 (their signal interferes with the detectors of the SP5 and SP8 microscopes).
  • Wipe off dust and smear from the lens and the sample coverslip using lens tissue (soaked in p.a. ethanol if required).
  • Login with your q-account onto the PC (thus you also get access to your usual group network drives)

While working:

  • Check which immersion media are required for each lens you use (oil, glycerol, water, air).
  • Use moderate amounts (1 drop=50 µl) of immersion media. Immediately remove immersion media spilling over the objective rim.
  • Create error logs when asked (software crash) and save the files under D:/errorlogs

After you have finished:

  • Finish with your experiment 5 min before the scheduled end of your booked slot!
  • Clean immersion media from every lens you used with a dry lens tissue clean both the lens and the outer parts of the objective
  • One main aspect in this context: switching lasers and lamps off and on within short intervals drastically reduces their lifetime!
  • 2-hours-rule:Check whether the system is booked within 2 hours on the same day.
    • If you are second last to use the system, make sure the user after you shows up.
    • The argon laser and the fluorescence lamp must be left on (in STANDBY mode), if the pause between two slots is 2 hours or less. (lasers that are not needed within the next 2 hours can be switched off)
    • If there is no other user within 2 hours after you have stopped, switch off all lamps and lasers as described in the short-manual for each system provided on the website.
    • ALWAYS INFORM FRIDAY AFTERNOON-/ EVENING-USERS that the system has been left on for them.
  • STANDBY mode (new user within next 2 hours!!): see detailed instructions for the respective system (SP5)
  • OFF mode (no user within next 2 hours/overnight/over weekend!!!): see detailed instructions for the respective system (SP5)
  • Transfer your data and remove them from the harddisk
  • Comment on the performance of the system by writing into the logbook(e.g., o.k. or a failure description).
    • Be concrete, short and avoid emotions.
    • In case of software errors write an errorlog.

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