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SP5 STARTUP AND SHUTDOWN

These short startup and shut-down instructions do not replace the mandatory personal introduction of each user to the system! 

In oder to improve the efficiency the incubation box will continously be operated at 37°C each week from Wednesday to Friday and at room temperature Mondays and Tuesdays.

Therefore fixed cells should be analyzed Monday or Tuesday, and live cells from Wednesday to Friday. This rule will avoid loss of instrument time due to lenghty warmup and cool-down times, each requiring a full hour. CO2 levels can be changed within relatively short time, therefore CO2 will not be permanently activated.

On weekends users are allowed to choose the temperature.

STARTUP FROM OFF MODE
  • Switch on the room air condition with the remote control; temperature 23°C may not be changed (optimal for SP5)
  • Incubation box will be set to 37°C from Wednesday to Friday.
  • Switch on the fluorescence lamp: left lower side.
  • Check insided the incubation box: no items may block the movement of the automated stage (DANGER OF DAMAGING THE INSTRUMENT!)
  • Three green buttons plus one key: right side of table, control box
    • Switch on leftmost of green switches: "PC/Microscope"
    • Middle switch: Scanner ON
    • Right switch: Lasers ON: Ventilation system starts!!
    • Turn key to ON position
  • Log on Windows using your q-number and password
  • Startup imaging software: LASAF
    • First startup window: Tick resonant scanner box, if required (fast FRAP,....) otherwise standard scanner will be used (most experiments!).
    • Second startup window: "initialize automated stage". Before selecting yes, make once again sure that there are no items blocking the movement of the stage; it will move over the entire possible range if "yes" is selected. If you take "no" the stage will still work but it will not communicate with the software (no storage and refinding of positions!)
    • After the second startup window  the software shoud start without further delay.
    • Activate required lasers in the configuration menu and proceed with experiment.
SWITCHING TO STANDBY MODE (next user within 2 hours)
  • Transfer your data to your network drive and remove them from the hard disk
  • Clean objectives from any immersion medium using lens tissue paper
    • Objectives should be completely clean, no visible traces of immersion media! (water is easiest, glycerol is water soluble, oil may be removed using some EtOH)
    • Also clean the outer parts of the objectives.
  • Check which lasers are required by the next user (booking), leave those ON!
  • Argon laser: if left on for the next user, power should be set to minimum output (0%) in the configuration menu.
  • Incubation boxand CO2: Temperature leave for next user.
    • close CO2 valve (flask) if not required anymore
  • Fluorescence lamp: leave on.
  • Close software.
  • Logoff from Windows, but don't shut down the PC.
  • Leave on the air condition.
  • MAKE SURE THAT THE NEXT USER REALLY SHOWS UP (wait or phone!)
  • Comment on the performance in the logbook
SHUTDOWN TO OFF MODE (no user within 2 h, overnight, weekend)
  • Switch off lasers in the software (configuration menu, tick boxes)
  • Close software
  • Clean objectives from any immersion medium using lens tissue paper
    • Objectives should be completely clean, no visible traces of immersion media! (water is easiest, glycerol is water soluble, oil may be removed using some EtOH)
    • Also clean the outer parts of the objectives
  • Three green buttons plus one key: right side of table, control box
    • Switch off "Scanner"
    • Turn key to "OFF" position
    • Leave ON the right green button "Lasers" until cooling has finished (i.e. ventilation has switched off and no warm air is blown out anymore).
    • Transfer your data to your network drive and remove them from the hard disk in the meantime
    • ONLY NOW switch off the green button "Laser" (no fan noise and no air coming out of the "black box" in the right corner)
  • ANY FAILURE TO FOLLOW THE LASER SHUT DOWN PROCEDURE CAN RESULT IN SERIOUS DAMAGE TO THE INSTRUMENT (NO EXCUSES!)
  • Switch off the fluorescence lamp (left side!)
  • Shut down Windows
  • Switch off green button "PC/System"
  • Switch off the CO2 on the incubation box 
  • CO2: Close the valve on the flask
  • Cover instrument with plastic cover
  • Recheck that you have not forgotten anything (especially the fluorescence lamp!)
  • Comment on the performance in the logbook
STARTUP FROM STANDBY MODE
  • Log on Windows using your q-number and password
  • Startup imaging software: LASAF
    • First startup window: Tick resonant scanner box, if required (fast FRAP, 4D imaging,...) otherwise standard scanner will be used (most experiments!).
    • Second startup window: "initialize automated stage". Before selecting yes, make once again sure that there are no items blocking the movement of the stage; it will move over the entire possible range if "yes" is selected. If you take "no" the stage will still work but it will not communicate with the software (no storage and refinding of positions!)
    • After the second startup window  the software should start with little further delay.
    • Activate additional required lasers in the configuration menu and proceed with experiment

 

 

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