Protocols

On this page I will publish protocols for microscopy in pdf format.

Objective_correction : Summary how to optimize the objectives using the correction collars!
SP5 image data: How to extraxt image infos from lif files
SP5_errorLog: How to generate an error report on the SP5 microscope
Perfusion system: How to add substances, replace medium, without "on scope pipetting"!
Leica protocols for the SP5 microscope:

Dye separation: How to seperate dyes with a strong fluorescence overlap-linear unmixing.
FRAP: How to perform FRAP and photoactivation experiments.
FRET:FRET acceptor bleaching and FRET sensitized emission wizards.
Live data mode: usage of the "live data mode" to combine different time series, multiposition imaging,..."programming" the SP5 scope.
Glycerol objective: information on the extra-wide working distance glycerol objective-recommended for thick samples such as brain sections,...

SP5_deconvolution: Instructions how to aquire images for deconvolution using the Huygens software.